Course Information

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
In-person direct teaching

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 5/36, only registered with preference (fields directly associated with the programme): 5/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western blotting
• Work with protein databases
• Determining 3D conformation of proteins with similar AA sequence

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: in blocks.

Teacher's information

All the tasks of the practical course will be taught in a week-long block, if not announced differently, then always in the last week of a given semester.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Ing. Renata Holánková (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 14/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western blotting
• Work with protein databases
• Determining 3D conformation of proteins with similar AA sequence

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

Teacher's information

All the tasks of the practical course will be taught in a week-long block, if not announced differently, then always in the last week of a given semester.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
Ing. Eliška Kadlecová (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Kateřina Konečná (assistant)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 2/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western blotting
• Work with protein databases
• Determining 3D conformation of proteins with similar AA sequence

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

Teacher's information

All the tasks of the practical course will be taught in a week-long block, if not announced differently, then always in the last week of a given semester.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 1/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein preparation using different techniques and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting
• Work with protein databases
• Determination of 3D conformation of proteins with similar amino acid sequence

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

Teacher's information

All the tasks of the practical course will be taught in a week-long block, if not announced differently, then always in the last week of a given semester.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Ing. Eliška Kadlecová (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Lucia Tomovičová (assistant)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Agnieszka Szmitkowska, Ph.D., M.Sc. (seminar tutor)
Ing. Eliška Kadlecová (assistant)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

CG090 Proteomic methods ||NOW( CG090 Proteomic methods )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.

Learning outcomes

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
Danuše Fridrichová (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Agnieszka Szmitkowska, Ph.D., M.Sc. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

C8302 Proteomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Bc. Martina Válková (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Agnieszka Szmitkowska, Ph.D., M.Sc. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

C8302 Proteomics - practice

Extent and Intensity

0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (assistant)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Bc. Martina Válková (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable of Seminar Groups

C8302/Beh_01: No timetable has been entered into IS.
C8302/Beh_02: No timetable has been entered into IS.

Prerequisites (in Czech)

C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

C8302 Proteomics - practice

Extent and Intensity

0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Philip Anthony P. Jackson, Ph.D. (lecturer)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Šárka Michlíčková (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Agnieszka Szmitkowska, Ph.D., M.Sc. (seminar tutor)
Bc. Martina Válková (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites (in Czech)

C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.

C8302 Proteomics - practice

Extent and Intensity

0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (seminar tutor)
Bc. Martina Válková (seminar tutor)
Philip Anthony P. Jackson, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Agnieszka Szmitkowska, Ph.D., M.Sc. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable of Seminar Groups

C8302/C8302_beh_01: No timetable has been entered into IS. J. Hejátko, P. Jackson, H. Konečná, G. Lochmanová, Š. Michlíčková, A. Szmitkowska, M. Válková, Z. Zdráhal
C8302/C8302_beh_02: No timetable has been entered into IS. J. Hejátko, P. Jackson, H. Konečná, G. Lochmanová, Š. Michlíčková, A. Szmitkowska, M. Válková, Z. Zdráhal

Prerequisites (in Czech)

C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

C8302 Proteomics - practice

Extent and Intensity

0/3. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: graded credit, z (credit).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Bc. Martina Válková (seminar tutor)
Mgr. Zuzana Gelová, Ph.D. (seminar tutor)
Ing. Matej Zábrady, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (assistant)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable of Seminar Groups

C8302/C8302_Beh_01: No timetable has been entered into IS. R. Dopitová, Z. Gelová, J. Hejátko, T. Klumpler, H. Konečná, G. Lochmanová, Š. Michlíčková, M. Válková, M. Zábrady, Z. Zdráhal
C8302/C8302_Beh_02: No timetable has been entered into IS. R. Dopitová, Z. Gelová, J. Hejátko, T. Klumpler, H. Konečná, G. Lochmanová, Š. Michlíčková, M. Válková, M. Zábrady, Z. Zdráhal

Prerequisites (in Czech)

C8202 Proteomics || CG010 Proteomics ||NOW( C8202 Proteomics )||NOW( CG010 Proteomics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

C8302 Proteomics - practice

Extent and Intensity

0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (assistant)
Bc. Martina Válková (seminar tutor)
Mgr. Zuzana Gelová, Ph.D. (seminar tutor)
Ing. Matej Zábrady, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable of Seminar Groups

C8302/C8302_01: No timetable has been entered into IS. J. Hejátko, H. Konečná, G. Lochmanová, Z. Zdráhal
C8302/C8302_02: No timetable has been entered into IS.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB)

Course objectives

Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.

Syllabus

• Two-dimensional electrophoresis of proteins
• Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
• Preparation, isolation and sequencing of recombinant DNA
• Expression of recombinant DNA in E.coli
• Single and multistep purification of recombinant proteins
• Purity determination and analysis of recombinant proteins
• Western bloting

Literature

• THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
• LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.

Assessment methods

The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

• Enrolment Statistics (recent)