Course Information

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
In-person direct teaching

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
prof. Mgr. Martin Lysák, Ph.D., DSc. (lecturer)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
doc. Mgr. Petra Procházková Schrumpfová, Ph.D. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 9:00–10:50 B11/335

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
prof. Mgr. Martin Lysák, Ph.D., DSc. (lecturer)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 10:00–11:50 B11/335

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
prof. Mgr. Martin Lysák, Ph.D., DSc. (lecturer)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
prof. Mgr. Martin Lysák, Ph.D., DSc. (lecturer)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 9:00–10:50 B11/305

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
prof. Mgr. Martin Lysák, Ph.D., DSc. (lecturer)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 10:00–11:50 prace doma

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)
Mgr. Pavla Pospíšilová (assistant)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 9:00–10:50 B11/305

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory.

Learning outcomes

At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)
doc. Mgr. Jan Havliš, Dr. (assistant)
Mgr. Pavla Pospíšilová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 17. 9. to Fri 14. 12. Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written and oral exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F. The student must complete successfully (i.e. evaluation better than F) the written test to be allowed to proceed with the oral exam. The final grade will consist of both written test evaluation (weight 0.6) and oral exam grade (weight 0.4).

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)
Mgr. Pavla Pospíšilová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 18. 9. to Fri 15. 12. Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written and oral exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F. The student must complete successfully (i.e. evaluation better than F) the written test to be allowed to proceed with the oral exam. The final grade will consist of both written test evaluation (weight 0.6) and oral exam grade (weight 0.4).

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)
Mgr. Pavla Pospíšilová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 19. 9. to Sun 18. 12. Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (C7301), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• C7301 Genomics - practice
• C8202 Proteomics - a basic course
• S2008 Developmental and cellular biology of plants

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)
Mgr. Pavla Pospíšilová (assistant)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (Bi7201c), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• Bi7201c Genomics - practice

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 8:00–9:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (Bi7201c), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• Bi7201c Genomics - practice

Further comments (probably available only in Czech)

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 12:00–13:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (Bi7201c), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analysis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analysis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analysis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitative gene expression profiling, Genevestigator, translational and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Literature

recommended literature
• SAMUELSSON, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. Cambridge: Cambridge University Press, 2012, xvii, 338. ISBN 9781107008564. info
• Genomics. Edited by Isidore Rigoutsos - G. Stephanopoulos. New York: Oxford University Press, 2007, xxi, 314 p. ISBN 9780195300819. info

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials are available on-line. Type of exam: Written exam. The test consists from 40 questions valued according to their difficulties (1-4 points), the total maximum is 100 points. Evaluation criteria of the examination test are as follows: 100-95 points A, 94-88 points B, 87-80 points C, 79-70 points D, 69-58 points E, <58 points F.

Language of instruction

Czech

Follow-Up Courses

• Bi7201c Genomics - practice

Further Comments

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer), prof. RNDr. Jiří Fajkus, CSc. (deputy)
RNDr. Roman Hobza, Ph.D. (lecturer), doc. RNDr. Jan Hejátko, Ph.D. (deputy)
RNDr. Hana Konečná (lecturer)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Mgr. Kamil Růžička, Dr. rer. nat. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Fri 8:00–9:50 B11/306

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, N-BCH)
• Genomics and Proteomics (programme PřF, D-BI4)
• Genomics and Proteomics (programme PřF, N-BCH)
• Environmental Chemistry (programme PřF, N-CH)

Course objectives

At the end of the course, student will acquire theory of basic approaches in current functional genomics: Theory of basic bioinformatics tools, basics of the work with genomic databases, identification of gene function using in silico approach, targeted gene silencing and gain of function approaches, phenotype profiling (DNA, RNA and protein chips), identification and analysis of sequence specific mutants, fragmental analysis and positional cloning, etc.. Lectures are conceived as an extended introduction into practical courses (Bi7201c), in frame of which students will be able to practice most of the theory. At the end of this course, students get an overview of modern approaches of functional genomics. Students will be able to use and interpret information deposited in genomic databases and they will be oriented in the modern biology approaches and problems. Student will be able to contribute in a creative way to the further development of modern biology.

Syllabus

• Introduction to genomics.
• Methods of functional genomics.
• Genome databases and basic tools of bioinformatics: (types of databases, databases search, finding of similar sequences [BLAST and FASTA], multiple sequence alignment [CLUSTALW], search in genomic databases of Arabidopsis thaliana, localization of genes on chromozomes, identification and analyzis of promoter regions of individual genes [ALIBABA], virtual PCR).
• In silico prediction of gene functions.
• Forward and reverse genetics approaches (methods of the identification of sequence specific mutants, mutant collections and their analyzis, physical and chemical mutagenesis, targeted gene silencing using RNA interference).
• Fragmental DNA analyzis and positional cloning as tools in forward genetics.
• Gain of function approaches in identification of the gene function (activation T-DNA mutagenesis, ectopic overexpression, regulated gene expresion).
• Phenotype profiling (cDNA, RNA and protein chips, metabolic profiling, microdissection, proteomic approaches).
• Southern blot and DNA molecular hybridization.
• Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
• Methods for the gene expression analysis (qualitative and quantitaive gene expression profiling, Genevestigator, transaltional and transcriptional fusions with reporter gene).
• New approaches: Chemical genetics.

Teaching methods

The main teaching method are lectures that include particular examples from the own scientific praxis and demonstration of the solution of particular problems via individual tools of functional genomics.

Assessment methods

Type of lectures: The lectures are facultative, however, the presence of students is highly desirable for complete understanding of principles of functional genomics approaches; the teaching materials available on-line are rather supporting. Type of exam: Written exam.

Language of instruction

Czech

Follow-Up Courses

• Bi7201c Genomics - practice

Further Comments

Study Materials

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

Teacher's information

http://genpro.sci.muni.cz/

CG020 Genomics

The course is not taught in Autumn 2011

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Jakub Horák, Dr. rer. nat. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is offered to students of any study field.

Syllabus (in Czech)

• Základy bioinformatiky
• Teorie základních bioinformatických nástrojů: globální vs. lokální přiřazení ( Dot-plot, BLASTa jeho modifikace, FASTA), konstrukce genových map včetně (detekce ORFs, restrikčních míst, …). Základy práce s genomovými databázemi.
• Identifikace genů
• Postupy a rozdíly v základních myšlenkových dogmatech reverzní a přímé genetiky. Identifikace genové funkce: in silico (struktura genů a jejich vyhledávání pomocí software pro detekci exonů a míst sestřihu, genomová kolinearita a genová homologie), experimentální identifikace genů (technologie metylačního filtrování, EST knihovny), cíleným umlčováním genů a přístupy získané funkce (T-DNA mutageneze, ektopická nadměrná exprese)
• Přístupy reverzní genetiky
• Metody identifikace a analýzy sekvenčně specifických mutantů (příprava sbírky mutantů, vyhledávání sekvenčně specifických mutantů pomocí PCR, vyhledávání sekvenčně specifických mutantů v elektronických databázích), analýza fenotypu a potvrzení příčinné souvislosti mezi fenotypem a inzerční mutací (kongregační analýza, identifikace nezávislé alely, využití nestabilních inzerčních mutagenů a izolace revertantech linií). Umlčování genů pomocí RNAi (mechanismus účinku RNAi, příprava konstruktů pro umlčování genů pomocí RNAi)
• Přístupy genetiky přímé
• Využití knihoven inzerčních mutantů v postupech přímé genetiky; vyhledávání v knihovnách inzerčních mutantů podle (vnějšího fenotypu, metabolického profilu, exprese genů zájmu); identifikace mutovaného lokusu (plasmid rescue, inverzní PCR). Využití knihoven bodových mutantů v přímé genetice: Fragmentační analýza a poziční klonování
• Přístupy funkční genomiky
• Analýza genové exprese (metody kvalitativní i analýzy genové exprese, transkripční vs. translační fúze, metody regulace transgenů). Metody identifikace funkce genů pomocí přístupů získané funkce (T-DNA aktivační mutageneze, ektopická exprese a systémy regulovatelné genové exprese). Fenotypové profilování (DNA a proteinové čipy, metabolické profilování, metody mikrodisekce). Metody využívané ve funkční genomice rostlin (A. thaliana jako modelový organizmus funkční genomiky rostlin, příprava transgenních rostlin, Southern blot a DNA molekulární hybridizace, izolace genomové DNA, PCR). Nové trendy (chemická genetika).
• Strukturní genomika
• Základní strukturní rysy organizace genomů jednotlivých organizmů včetně člověka (viry, eubakterie, kvasinky, vyšší a nižší rostliny, obratlovci). Analýza protein-proteinových interakcí: Význam interakcí NA a proteinů v živých systémech: TF, telomeráza, nukleozómy. Kódující a nekódující DNA: Strukturní geny, RNA geny (rRNA, pre-miRNA a shRNA), transpozony.
• Moderní postupy funkční genomiky
• Next-gen sequencing a jeho využití v genomice (analýza genové exprese, identifikace SNPs, metagenomika). Tkáňově specifická analýza genová exprese (buněčně specifické GFP linie a buněčné sortování, mikrodisekce).
• Úloha lokalizace genových produktů v buňce
• GFP fůze, imunolokalizace, moderní metody detekce (FLIM, FRAP, fotoaktivovatelné proteiny, FCS).
• Praktické aplikace funkční genomiky
• Šlechtění rostlin, individualizovaná medicína, pokročilé biotechnologie (produkce protilátek v rostlinách (imunomodulace), produkce terapeuticky využitelných látek, doplňky stravy). Význam a bezpečnost GMO.

Teaching methods (in Czech)

výuka je založena na ppt prezentacích a jejich výkladu.

Assessment methods (in Czech)

zkouška ústní

Language of instruction

Czech

Further Comments

The course is taught: every week.

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

CG020 Genomics

The course is not taught in Autumn 2010

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Jakub Horák, Dr. rer. nat. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Chemistry (programme PřF, N-CH)
• Biochemistry (programme PřF, N-BCH)
• Genomics and Proteomics (programme PřF, D-BI4)

Syllabus (in Czech)

• Základy bioinformatiky
• Teorie základních bioinformatických nástrojů: globální vs. lokální přiřazení ( Dot-plot, BLASTa jeho modifikace, FASTA), konstrukce genových map včetně (detekce ORFs, restrikčních míst, …). Základy práce s genomovými databázemi.
• Identifikace genů
• Postupy a rozdíly v základních myšlenkových dogmatech reverzní a přímé genetiky. Identifikace genové funkce: in silico (struktura genů a jejich vyhledávání pomocí software pro detekci exonů a míst sestřihu, genomová kolinearita a genová homologie), experimentální identifikace genů (technologie metylačního filtrování, EST knihovny), cíleným umlčováním genů a přístupy získané funkce (T-DNA mutageneze, ektopická nadměrná exprese)
• Přístupy reverzní genetiky
• Metody identifikace a analýzy sekvenčně specifických mutantů (příprava sbírky mutantů, vyhledávání sekvenčně specifických mutantů pomocí PCR, vyhledávání sekvenčně specifických mutantů v elektronických databázích), analýza fenotypu a potvrzení příčinné souvislosti mezi fenotypem a inzerční mutací (kongregační analýza, identifikace nezávislé alely, využití nestabilních inzerčních mutagenů a izolace revertantech linií). Umlčování genů pomocí RNAi (mechanismus účinku RNAi, příprava konstruktů pro umlčování genů pomocí RNAi)
• Přístupy genetiky přímé
• Využití knihoven inzerčních mutantů v postupech přímé genetiky; vyhledávání v knihovnách inzerčních mutantů podle (vnějšího fenotypu, metabolického profilu, exprese genů zájmu); identifikace mutovaného lokusu (plasmid rescue, inverzní PCR). Využití knihoven bodových mutantů v přímé genetice: Fragmentační analýza a poziční klonování
• Přístupy funkční genomiky
• Analýza genové exprese (metody kvalitativní i analýzy genové exprese, transkripční vs. translační fúze, metody regulace transgenů). Metody identifikace funkce genů pomocí přístupů získané funkce (T-DNA aktivační mutageneze, ektopická exprese a systémy regulovatelné genové exprese). Fenotypové profilování (DNA a proteinové čipy, metabolické profilování, metody mikrodisekce). Metody využívané ve funkční genomice rostlin (A. thaliana jako modelový organizmus funkční genomiky rostlin, příprava transgenních rostlin, Southern blot a DNA molekulární hybridizace, izolace genomové DNA, PCR). Nové trendy (chemická genetika).
• Strukturní genomika
• Základní strukturní rysy organizace genomů jednotlivých organizmů včetně člověka (viry, eubakterie, kvasinky, vyšší a nižší rostliny, obratlovci). Analýza protein-proteinových interakcí: Význam interakcí NA a proteinů v živých systémech: TF, telomeráza, nukleozómy. Kódující a nekódující DNA: Strukturní geny, RNA geny (rRNA, pre-miRNA a shRNA), transpozony.
• Moderní postupy funkční genomiky
• Next-gen sequencing a jeho využití v genomice (analýza genové exprese, identifikace SNPs, metagenomika). Tkáňově specifická analýza genová exprese (buněčně specifické GFP linie a buněčné sortování, mikrodisekce).
• Úloha lokalizace genových produktů v buňce
• GFP fůze, imunolokalizace, moderní metody detekce (FLIM, FRAP, fotoaktivovatelné proteiny, FCS).
• Praktické aplikace funkční genomiky
• Šlechtění rostlin, individualizovaná medicína, pokročilé biotechnologie (produkce protilátek v rostlinách (imunomodulace), produkce terapeuticky využitelných látek, doplňky stravy). Význam a bezpečnost GMO.

Teaching methods (in Czech)

výuka je založena na ppt prezentacích a jejich výkladu.

Assessment methods (in Czech)

zkouška ústní

Language of instruction

Czech

Further Comments

The course is taught: every week.

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

CG020 Genomics

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Jakub Horák, Dr. rer. nat. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is offered to students of any study field.

Syllabus (in Czech)

• Základy bioinformatiky
• Teorie základních bioinformatických nástrojů: globální vs. lokální přiřazení ( Dot-plot, BLASTa jeho modifikace, FASTA), konstrukce genových map včetně (detekce ORFs, restrikčních míst, …). Základy práce s genomovými databázemi.
• Identifikace genů
• Postupy a rozdíly v základních myšlenkových dogmatech reverzní a přímé genetiky. Identifikace genové funkce: in silico (struktura genů a jejich vyhledávání pomocí software pro detekci exonů a míst sestřihu, genomová kolinearita a genová homologie), experimentální identifikace genů (technologie metylačního filtrování, EST knihovny), cíleným umlčováním genů a přístupy získané funkce (T-DNA mutageneze, ektopická nadměrná exprese)
• Přístupy reverzní genetiky
• Metody identifikace a analýzy sekvenčně specifických mutantů (příprava sbírky mutantů, vyhledávání sekvenčně specifických mutantů pomocí PCR, vyhledávání sekvenčně specifických mutantů v elektronických databázích), analýza fenotypu a potvrzení příčinné souvislosti mezi fenotypem a inzerční mutací (kongregační analýza, identifikace nezávislé alely, využití nestabilních inzerčních mutagenů a izolace revertantech linií). Umlčování genů pomocí RNAi (mechanismus účinku RNAi, příprava konstruktů pro umlčování genů pomocí RNAi)
• Přístupy genetiky přímé
• Využití knihoven inzerčních mutantů v postupech přímé genetiky; vyhledávání v knihovnách inzerčních mutantů podle (vnějšího fenotypu, metabolického profilu, exprese genů zájmu); identifikace mutovaného lokusu (plasmid rescue, inverzní PCR). Využití knihoven bodových mutantů v přímé genetice: Fragmentační analýza a poziční klonování
• Přístupy funkční genomiky
• Analýza genové exprese (metody kvalitativní i analýzy genové exprese, transkripční vs. translační fúze, metody regulace transgenů). Metody identifikace funkce genů pomocí přístupů získané funkce (T-DNA aktivační mutageneze, ektopická exprese a systémy regulovatelné genové exprese). Fenotypové profilování (DNA a proteinové čipy, metabolické profilování, metody mikrodisekce). Metody využívané ve funkční genomice rostlin (A. thaliana jako modelový organizmus funkční genomiky rostlin, příprava transgenních rostlin, Southern blot a DNA molekulární hybridizace, izolace genomové DNA, PCR). Nové trendy (chemická genetika).
• Strukturní genomika
• Základní strukturní rysy organizace genomů jednotlivých organizmů včetně člověka (viry, eubakterie, kvasinky, vyšší a nižší rostliny, obratlovci). Analýza protein-proteinových interakcí: Význam interakcí NA a proteinů v živých systémech: TF, telomeráza, nukleozómy. Kódující a nekódující DNA: Strukturní geny, RNA geny (rRNA, pre-miRNA a shRNA), transpozony.
• Moderní postupy funkční genomiky
• Next-gen sequencing a jeho využití v genomice (analýza genové exprese, identifikace SNPs, metagenomika). Tkáňově specifická analýza genová exprese (buněčně specifické GFP linie a buněčné sortování, mikrodisekce).
• Úloha lokalizace genových produktů v buňce
• GFP fůze, imunolokalizace, moderní metody detekce (FLIM, FRAP, fotoaktivovatelné proteiny, FCS).
• Praktické aplikace funkční genomiky
• Šlechtění rostlin, individualizovaná medicína, pokročilé biotechnologie (produkce protilátek v rostlinách (imunomodulace), produkce terapeuticky využitelných látek, doplňky stravy). Význam a bezpečnost GMO.

Teaching methods (in Czech)

výuka je založena na ppt prezentacích a jejich výkladu.

Assessment methods (in Czech)

zkouška ústní

Language of instruction

Czech

Further Comments

The course is taught: every week.

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

CG020 Genomics

The course is not taught in Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Jakub Horák, Dr. rer. nat. (lecturer)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Chemistry (programme PřF, N-CH)
• Biochemistry (programme PřF, N-BCH)
• Genomics and Proteomics (programme PřF, D-BI4)

Syllabus (in Czech)

• Základy bioinformatiky
• Teorie základních bioinformatických nástrojů: globální vs. lokální přiřazení ( Dot-plot, BLASTa jeho modifikace, FASTA), konstrukce genových map včetně (detekce ORFs, restrikčních míst, …). Základy práce s genomovými databázemi.
• Identifikace genů
• Postupy a rozdíly v základních myšlenkových dogmatech reverzní a přímé genetiky. Identifikace genové funkce: in silico (struktura genů a jejich vyhledávání pomocí software pro detekci exonů a míst sestřihu, genomová kolinearita a genová homologie), experimentální identifikace genů (technologie metylačního filtrování, EST knihovny), cíleným umlčováním genů a přístupy získané funkce (T-DNA mutageneze, ektopická nadměrná exprese)
• Přístupy reverzní genetiky
• Metody identifikace a analýzy sekvenčně specifických mutantů (příprava sbírky mutantů, vyhledávání sekvenčně specifických mutantů pomocí PCR, vyhledávání sekvenčně specifických mutantů v elektronických databázích), analýza fenotypu a potvrzení příčinné souvislosti mezi fenotypem a inzerční mutací (kongregační analýza, identifikace nezávislé alely, využití nestabilních inzerčních mutagenů a izolace revertantech linií). Umlčování genů pomocí RNAi (mechanismus účinku RNAi, příprava konstruktů pro umlčování genů pomocí RNAi)
• Přístupy genetiky přímé
• Využití knihoven inzerčních mutantů v postupech přímé genetiky; vyhledávání v knihovnách inzerčních mutantů podle (vnějšího fenotypu, metabolického profilu, exprese genů zájmu); identifikace mutovaného lokusu (plasmid rescue, inverzní PCR). Využití knihoven bodových mutantů v přímé genetice: Fragmentační analýza a poziční klonování
• Přístupy funkční genomiky
• Analýza genové exprese (metody kvalitativní i analýzy genové exprese, transkripční vs. translační fúze, metody regulace transgenů). Metody identifikace funkce genů pomocí přístupů získané funkce (T-DNA aktivační mutageneze, ektopická exprese a systémy regulovatelné genové exprese). Fenotypové profilování (DNA a proteinové čipy, metabolické profilování, metody mikrodisekce). Metody využívané ve funkční genomice rostlin (A. thaliana jako modelový organizmus funkční genomiky rostlin, příprava transgenních rostlin, Southern blot a DNA molekulární hybridizace, izolace genomové DNA, PCR). Nové trendy (chemická genetika).
• Strukturní genomika
• Základní strukturní rysy organizace genomů jednotlivých organizmů včetně člověka (viry, eubakterie, kvasinky, vyšší a nižší rostliny, obratlovci). Analýza protein-proteinových interakcí: Význam interakcí NA a proteinů v živých systémech: TF, telomeráza, nukleozómy. Kódující a nekódující DNA: Strukturní geny, RNA geny (rRNA, pre-miRNA a shRNA), transpozony.
• Moderní postupy funkční genomiky
• Next-gen sequencing a jeho využití v genomice (analýza genové exprese, identifikace SNPs, metagenomika). Tkáňově specifická analýza genová exprese (buněčně specifické GFP linie a buněčné sortování, mikrodisekce).
• Úloha lokalizace genových produktů v buňce
• GFP fůze, imunolokalizace, moderní metody detekce (FLIM, FRAP, fotoaktivovatelné proteiny, FCS).
• Praktické aplikace funkční genomiky
• Šlechtění rostlin, individualizovaná medicína, pokročilé biotechnologie (produkce protilátek v rostlinách (imunomodulace), produkce terapeuticky využitelných látek, doplňky stravy). Význam a bezpečnost GMO.

Teaching methods (in Czech)

výuka je založena na ppt prezentacích a jejich výkladu.

Assessment methods (in Czech)

zkouška ústní

Language of instruction

Czech

Further Comments

The course is taught: every week.

Listed among pre-requisites of other courses

• C7301 Genomics - practice
  NOW(C7201) || NOW(CG020)  

• Enrolment Statistics (recent)