PřF:C8160 Enzymology - Course Information
C8160 Enzymology
Faculty of ScienceSpring 2008
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- prof. RNDr. Igor Kučera, DrSc. (lecturer)
- Guaranteed by
- prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 14:00–15:50 C05/114
- Prerequisites
- C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry, organic chemistry and physical chemistry are recommended. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, D-CH) (2)
- Inorganic Chemistry (programme PřF, D-CH) (2)
- Biochemistry (programme PřF, D-CH) (2)
- Biochemistry (programme PřF, M-CH)
- Biochemistry (programme PřF, N-CH)
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Physical Chemistry (programme PřF, D-CH) (2)
- Macromolecular Chemistry (programme PřF, D-CH) (2)
- Environmental Chemistry (programme PřF, D-CH) (2)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Organic Chemistry (programme PřF, D-CH) (2)
- Course objectives
- An advanced lecture. Enzyme nomenclature. Measurement of enzyme activity. Isolation and storage of enzymes. Mechanisms of enzyme catalysis. Enzyme kinetics. The effect of temperature, pH and inhibitors. Techniques of enzyme immobilization, the effects of immobilization on enzyme properties. Use of enzymes in biochemical analyses,molecular biology, medicine and technology.
- Syllabus
- 1) First sight into the world of enzymes: structure, specifity, nomenclature. 2) Enzyme activity and their measurement. 3) Isolation of enzymes from the natural sources and expression systems. 4) Enzyme catalysis from the point of view of organic chemistry. 5) Enzyme catalysis from the point of view of physical chemistry. 6) Basic theoretical concepts in enzyme kinetics. 7) Effects of environmental factors (pH, temperature) on enzymes. 8) Reversible and irreversible inhibition of enzymes. 9) Kinetics of multisubstrate enzyme reactions. 10) Cooperativity in enzyme function. 11) Immobilized enzyme systems. 12) Enzymes as tools in bioanalysis. 13) Use of enzymes in technology and medicine. Enzyme engineering.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- KOTYK, Arnošt and Jaroslav HORÁK. Enzymová kinetika. Vyd. 1. Praha: Academia, 1977, 268 s. info
- Assessment methods (in Czech)
- Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2008, recent)
- Permalink: https://is.muni.cz/course/sci/spring2008/C8160