PřF:C8155 Dynamic biochem. II - signal. - Course Information

C8155 Dynamic biochemistry II - signaling pathways

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. RNDr. Stanislav Pavelka, CSc. (lecturer)

Guaranteed by

doc. RNDr. Stanislav Pavelka, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Stanislav Pavelka, CSc.

Timetable

Tue 10:00–11:50 C05/114

Prerequisites

Could be registered in the 3rd, 5th, 7th or 9th semester (after completing Biochemie II)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 17 fields of study the course is directly associated with, display

Course objectives

Dynamic biochemistry II - signaling pathways. A follow-up lecture course of dynamic biochemistry, pathobiochemistry and biochemical control of complex physiological processes for MSc (and also PhD) and Bc students of biochemistry and molecular biology and also of general biology, chemistry and medicine.

Main objectives of the course can be summarized as follows:
to extend the knowledge of the basic biochemical control mechanisms at the molecular level;
to get a view of the main signal transduction pathways occurring in animal cells;
to analyze the signaling mechanisms engaged in the intracellular and intercellular communication;
to learn new information on biomedical aspects of biochemistry.

Overview of basic types of signaling molecules, receptors, effectors, second messengers, and protein kinases. Mechanisms of signal transduction processes mediated by surface receptors and signaling through intracellular receptors. Interaction of different signal transduction pathways in the control of complex physiological processes.

Syllabus

• 1. Types of signaling molecules and intercellular signal transduction processes in eukaryots. (Hormones, growth factors, neurotransmitters, cytokines. Endocrine, neurocrine, paracrine, and autocrine communications). 2. Classification of hormones according to the type of receptors and mechanism of their action. (Hierarchical arrangement of the endocrine system. Feedback regulation of the hormone secretion). 3. Metabolism of hormones and basic experimental methods in the study of their actions. (Biosynthesis of peptide and protein hormones. Biosynthesis of the thyroid hormones and their metabolism in tissues). 4. Mechanisms of signal transduction mediated by plasma membrane receptors. (Basic types of receptors, effectors, second messengers, and protein kinases. Amplification function of receptor-effector-second messenger-protein kinase cascade). 5. Heterotrimeric G-proteins and their functions. (Mechanism of signal transduction mediated by G-proteins. Basic types of G-proteins, alpha-, beta-, and gamma-subunits. Mechanism of the effects of cholera toxin and pertussis toxin. The use of non-hydrolyzable analogs of GTP in the study of G-proteins function). 6. The most important signal transduction pathways initiated by the interaction of extracellular ligands with the G-protein coupled receptors. (Adenylate cyclase cascade and the mechanism of activation of protein kinase A. Phosphoinositide cascade and activation of protein kinase C). 7. The role of intracellular calcium and calmodulin in signal transduction. (Inositoltrisphosphate and ryanodine receptors. Signaling properties of cADP-ribose. Mechanisms of calcium induced release of calcium). 8. Other signal transduction pathways - guanylate cyclases and NO synthases. 9. Receptor tyrosine kinases (RTKs) and MAP kinase cascade. (Mechanism of action of growth factors receptors. Src and other cytosolic tyrosine kinases. SH2 and SH3 binding domains. MAP kinase cascade). 10. Signaling through intracellular receptors. (Mechanism of action of steroid and thyroid hormone receptors). 11. Super-family of GTPases and their cellular functions. (The GTPase cycle, GNRF and GAP proteins. Comparison of the mechanism of action of the elongation factor EF-Tu and heterotrimeric G-proteins. Ras protein and products of other proto-oncogenes). 12. Adaptation of target cells - desensitization of receptors. "Receptor diseases" - disorders connected with impairments of signal transduction. 13. Convergence, divergence, and crosstalk of diverse signal transduction pathways. 14. Interaction of diverse signal transduction pathways in the regulation of complex physiological processes.

Literature

• KRAUSS, Gerhard. Biochemistry of Signal Transduction and Regulation. 4th enlarged ed. Weinheim: WILEY-VCH Verlag GmbH, 2008, 626 pp. ISBN 978-3-527-31397-6. info
• HELMREICH, E.J.M. The Biochemistry of Cell Signalling. Oxford: Oxford University Press, 2001, 328 pp. info
• GARRETT, R.H. and C.M. GRISHAM. Biochemistry, 2nd ed. Fort Worth, Orlando: Saunders College Publ., 1999, 1127 pp. info
• STRYER, L. Biochemistry, 4th ed. New York: Freeman and Co., 1995, 1064 pp. info
• VOET, D. and J.G. VOET. Biochemistry, 2nd ed. New York: J. Wiley & Sons, 1995, 1361 pp. info
• Ca. 200 blan k dispozici u přednášejícího.

Assessment methods

Lectures, abundantly documented with charts, diagrams, formulas and figures, which are offered to the students for making copies. Oral examination; 36 specified questions are at disposal.

Language of instruction

Czech

Follow-Up Courses

• C7150 Regulation of metabolism

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: Při týdenní výuce min. 8 posluchačů, při blokové bez omezení

• Enrolment Statistics (Spring 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2009/C8155