Bi0322c Specialized methods of pifgments and antioxidants analysis in plants - practice

Faculty of Science
Spring 2010
Extent and Intensity
0/2. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
Guaranteed by
prof. Ing. Miloš Barták, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
Course objectives
During practical courses, students will extract and quantify the pigments, glutathione and antioxidative enzymes from different plant material. The influence of stress conditions on analyzed compounds in plants will be demonstrated in easy experiments. Students will acquire basic methods of plant sample handling procedure and preparation. Last, but not least, students will learn advance operating of spectrophotometer and HPLC.
Syllabus
  • 1. Introduction. Safety in lab, requirement for successful termination.
  • 2. Pigments extraction from plant sample. Different extraction reagents.
  • 3. Thin-layer chromatography. Pigments absorbtion spectra.
  • 4. Preparation of standards from commercial prepared standards. Pigments standards analysis on HPLC.
  • 5. Evaluation of pigments chromatogrammes. Establishing of experiment Light conditions during the plant growth
  • 6. Sample preparations. Freeze-drying. Grinding. Rules during sample preparation.
  • 7. Study of the influence of low temperature on antioxidative enzyme activities. - I. catalase with oxygen electrode.
  • 8. Study of the influence of low temperature on antioxidative enzyme activities. – II. ascorbate peroxidase by spectrophotometer. Determination of protein amount by Bradford method.
  • 9. Sampling from the first experiment. Quantification of pigments spectrophotometrically.
  • 10. Pigments extractions from the first experiment. HPLC analysis.
  • 11. Extraction of glutathione from the first experiment and determination of its amount and redox state by HPLC.
  • 12. Extraction of ascorbate and determination of its amount and redox state by HPLC.
  • 13. Chromatogrammes analysis.
Assessment methods
group projects, homeworks final group project
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: na vyžádání možná výuka v angličtině.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2009, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2010/Bi0322c