IC041 Light Emitting Diodes in Chemical Analysis, Detection, Fluorescence Microscopy and Visualization

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
prof. RNDr. Miroslav Macka, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
This interdisciplinary short course is an intensive introduction to the properties and usage of light emitting diodes (LEDs) in chemical analysis and chemistry in general. The course will cover the background and theory and then will focus on practical issues and the applications of LEDs particularly for analytical optical methods (photometry, fluorimetry), optical detection in analytical flow-through methods (FIA, HPLC, CE), optical sensors, microfluidic chips, fluorescence microscopy and visualization in photochemistry and teaching. Examples of some elementary techniques used to characterise LEDs including measurement of emission spectra will be conducted as hands-on experiments. This is a both beginners and intermediate level course with respect to LEDs. The course is designed in such a way that a wide range of participants can benefit: those curious about the use of LEDs in chemistry and science, those starting to use LEDs, having some experience, and students, scientists, engineers or lecturers considering whether LEDs could be used in their specific work. The course is also suitable for persons involved in teaching in the areas of instrumental analytical chemistry, physical chemistry and photochemistry. The participants will benefit from some background in chemistry and analytical chemistry in particular, but this interdisciplinary course is designed in such a way that students or professionals with other background can participate and benefit as well. Prior exposure to and use of other LEDs is useful but not expected or necessary.
Syllabus
  • Lectures 1, 2 and 3 · Why use LEDs in chemistry and science? · History · Fundamentals (physical principles, construction) · Basics of LEDs in respect to their applications in analysis and chemistry · Application areas · Considerations in choosing a LED · Practical issues when using LEDs · Coupling of LEDs with optical fibers · Pulsed techniques: use of lock-in amplifiers and LEDs in TRF (time resolved fluorescence) Optical methods in chemical analysis with an emphasis on photometry and photometric detection, fluorimetry and optical detection methods in microfluidic chips · Basics of construction of simple LED-based photometers and fluorimeters · Fluorescence microscopy and visualization using a set-up for <$250 · Diode lasers for compact inexpensive LIF (Laser Induced Fluorescence) detection in on capillary and microfluidic chip formats · LEDs as light sources for photochemistry · Other advanced and specialized methods · Hands-on experiments: measuring emission spectra, optical power, intensity, transmittance, absorbance, pulsing LEDs and use of lock-in amplifier techniques · Workshop on examples from participants’ own specific usage, questions, discussion Lecture 4 · Workshop using examples incl. particular applications of course participants · Consultations
Teaching methods
Lectures
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught: in blocks.
Note related to how often the course is taught: v týdnu 29.3.-1.4.2010.
The course is also listed under the following terms Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.