S2002 Methods in plant cytogenetics and cytogenomics I. - practical course

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/24. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
RNDr. Terezie Malík Mandáková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Martin Lysák, Ph.D., DSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Terezie Malík Mandáková, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
Syllabus
  • Annotation This practical course will demonstrate classical as well as state-of-the-art cytogenetic techniques. The accent is put on cytogenetic methods beneficial to taxonomic, biosystematic and phylogeographic studies. Students will get acquainted with preparation of mitotic and meiotic chromosomes, chromosome counting, the course of male meiosis as well as with principles of light and fluorescence microscopy. Molecular cytogenetic techniques will include DNA probe labelling, fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and chromosome painting. Digital processing of captured chromosome images will be demonstrated. Syllabus Day 1 - introduction - preparation of mitotic chromosomes from root tip meristems (Nicotiana, Festuca x Lolium hybrids) and preparation of meiotic chromosome spreads from anthers - theoretical and practical principles of light and fluorescence microscopy - analysis of chromosome preparations using fluorescence microscopy (selection of suitable slides for Day 2) - direct and indirect labelling of DNA probes (amplification of the Arabidopsis-type telomere repeat by PCR; labelling of ribosomal DNA repeats, chromosome-specific BAC clones, and genomic DNA using nick translation) Day 2 - introduction - fluorescence in situ hybridization (FISH), chromosome painting, genomic in situ hybridization (GISH): PART I (slide pre-treatment, preparation of hybridization mix, probe denaturation) - stages of mitosis and meiosis (movie, meiosis analysed using phase and fluorescence microscopy, digital microscope photography) Day 3 - introduction - FISH, chromosome painting, GISH: PART II (fluorescence detection of hybridized DNA probes) - fluorescence microscopy, image capture - computer processing of microscope images (Adobe Photoshop) - conclusions
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.