IC113 Binding Analysis for Experimental Biologists

Faculty of Science
Spring 2014
Extent and Intensity
0/0. 2 credit(s) (plus 2 for the colloquium). Type of Completion: k (colloquium).
Teacher(s)
Petr Kuzmič, Dr. (lecturer), doc. Mgr. Ctirad Hofr, Ph.D. (deputy)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
Syllabus
  • LECTURE TOPICS 1. Principles of data analysis: Matching models to experimental data 2. Confidence intervals for rate and equilibrium constants 3. Time course of biochemical and biophysical processes: Mathematical models 4. Experimental methods for the determination of rate constants 5. Biochemical and biophysical equilibria: Mathematical models 6. Analysis of equilibrium binding data 7. Enzyme catalysis and inhibition of enzymes 8. Choosing the appropriate molecular mechanism based on available data Notes: • All lectures will include concrete examples from published literature
  • EXERCISES 1. Determination of an equilibrium binding constant for a protein-protein or protein-DNA interaction 2. Determination of rate constants for protein-ligand interaction 3. Determination of stoichiometry protein-ligand interaction 4. Selecting an appropriate mechanism for enzyme inhibition Notes: • Practical sessions will be held in the computer laboratory using the software DynaFit • All exercises will include actual data published in the literature • Students are encouraged to bring their own additional data
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught only once.
The course is taught: in blocks.
Note related to how often the course is taught: block lecture May 12 -16, 2014.
Teacher's information
http://www.biokin.com/dynafit/index.html
  • Enrolment Statistics (recent)
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