C6850 Chromatographic Methods II.

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems.
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 4.- 7. Hybrid electromigration/chromatographic separation methods. Ion progression in an electrical field, the basic equations, concepts, terms and parameters. Principles of electromigration separation, principles of elektrochromatographic methods. Capillary electrophoresis (CE), isotachophoresis (ITP), isoelectric focusing, capillary electrochromatography (CEC), micellar electrokinetic chromatography (MEKC), capillary gel electrophoresis, microemulsion electroinetic chromatography(MEEKC), transient isotachophoresis. Applications. Instrumentation.
  • 8.- 9. Inverse chromatography
  • 10.- 11. Hyphenated techniques in separation analysis.
  • 12. - 13. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
  • JPC - Journal of Planar Chromatography-Modern TLC, Akadémiai Kiadó, ISSN 0933-4173 (Print) 1789-0993 (Online)
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2015, recent)
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