PřF:Bi2060c Basic microbiology - practice - Course Information

Bi2060c Basic microbiology - practice

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
doc. RNDr. Miroslav Němec, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Krsek, CSc., MSc
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi2060c/01: Thu 9:00–9:50 E25/111, M. Krsek
Bi2060c/02: Tue 15:00–15:50 E25/111, M. Krsek
Bi2060c/03: Tue 16:00–16:50 E25/111, M. Krsek

Prerequisites (in Czech)

NOW( Bi2060 Basic microbiology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecological and Evolutionary Biology (programme PřF, B-EB)
• Ecological and Evolutionary Biology (programme PřF, B-EB, specialization Botany)
• Ecological and Evolutionary Biology (programme PřF, B-EB, specialization Zoology)

Course objectives

At the end of this course, students should be able to work aseptically, to inoculate and cultivate bacteria, to prepare stained and native microscopic slides, to determine number of bacteria in different samples, to isolate bacterial group from chosen samples, to work with yeasts, to use some basic identification methods.

Syllabus

• 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Determination of the concentration of antibiotics by microbiological methods. 7. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 12. Reports, test and microscopic examination/determination of unknown bacterial strain.

Literature

• JANDOVÁ, Božena a Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996. 67 s. ISBN 80-210-1374-5.

Teaching methods

Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.

Assessment methods

Active participation at practical classes, written reports, written test, credit

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• Bi4090c General microbiology - practical course
  NOW(Bi4090) && !NOWANY(Bi4091c, Bi5710c, Bi2060c) && !Bi4091c && !Bi5710c && !Bi2060c && rocnik(2)  
• Bi4091c Practise from general microbiology
  NOW(Bi4090) && !NOWANY(Bi4090c, Bi5710c, Bi2060c) && !Bi4090c && !Bi5710c && !Bi2060c && (2)  
• Bi5710c Microbiology - practice
  NOW(Bi5710) && !NOWANY(Bi4091c, Bi4090c, Bi2060c) && !Bi4091c && !Bi4090c && !Bi2060c && rocnik(2) || rocnik(3)  
• Bi7031cen Microbiology – Practical Course
  (!Bi5710c) && (!Bi4090c) && (!Bi2060c) && !NOWANY(Bi5710c,Bi4090c,Bi2060c)  

• Enrolment Statistics (Spring 2016, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2016/Bi2060c