Bi6400 Methods of molecular biology

Faculty of Science
spring 2018
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (spring 2018, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2018/Bi6400