Bi1130 Experimental embryology

Faculty of Science
Spring 2021
Extent and Intensity
0/2/0. 2 credit(s) (plus 1 for the colloquium). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Marcela Buchtová, Ph.D. (seminar tutor)
Mgr. Jakub Harnoš, Ph.D. (seminar tutor)
Mgr. Marek Hampl, Ph.D. (seminar tutor)
Mgr. Pavel Dobeš, Ph.D. (seminar tutor)
Mgr. Jana Hurychová (seminar tutor)
Mgr. Marie Šulcová, Ph.D. (seminar tutor)
Mgr. Nikodém Zezula (seminar tutor)
Guaranteed by
doc. RNDr. Marcela Buchtová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Marcela Buchtová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 7. 6. 8:00–12:00 D36/215, Tue 8. 6. 8:00–12:00 D36/215, Wed 9. 6. 8:00–12:00 D36/215, Thu 10. 6. 8:00–12:00 D36/215, Fri 11. 6. 8:00–12:00 D36/215
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course the students will get an overview of the most common experimental models used in experimental embryology and practical skills for their usage in variety of experiemntal approaches.
Learning outcomes
Students will be able to: - design simple experiments while using metods of experimental embryology - practically manage experiment on model species - determine developmental stages on models - describe significant microscopic structures in selected developmental stages in model species
Syllabus
  • 1. Introduction to the course - methodology of scientific work, experimental design, assignment of student projects
  • 2. Drosophila - work with embryos 3. C. elegans - work with embryos 4. Danio I - work with embryos
  • 5. Danio II - comparative microscopic embryology 6. Xenopus - work with embryos, microinjection
  • 7. Chicken model I - eggs opening, embryo staging,application of morphogen to chicken embryo (implantation, injection)
  • 8. Chicken model II - collection and processing of embryological material, skeleton staining, examples of developmental disorders
  • 9. Chicke model III - comparative microscopic embryology 10. Mouse model I - collection and processing of embryological material, establishment of primary cell and tissue cultures, transfection with GFP plasmid
  • 11. Mouse model II - LUC assay, kryosections, in vivo imaging on primary cell cultures
  • 12. Student project presentations
  • 13. Student project presentations
Literature
  • GILBERT, Scott F. Developmental biology. 9th ed. Sunderland, Mass.: Sinauer Associates, 2010, xxi, 711. ISBN 9780878935642. info
Teaching methods
Teaching is based on practical exercise on model species in laboratory. Moreover, students will discuss their projects, which will be design by them and presented to classmates. These presetations will be prepared by them individually at home. Microscopic slides will be demostrated first by teacher and then student will work with samples independently.
Assessment methods
Evaluation will be based on student performance during presentation of their project. Quality of written assay, presentation and discussion will be evaluated.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2021/Bi1130