PřF:B7722p Metody analýzy mikroorg. II -p

B7722p Speciální metody analýzy mikroorganizmů II. -p

Faculty of Science
Autumn 1999

Extent and Intensity

2/0/0. Type of Completion: -.

Teacher(s)

Guaranteed by

Prerequisites (in Czech)

Předpokladem zapsání je zkouška z předmětu Speciální metody analýzy mikroorganismů I (B6721).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the construction of DNA probes. Electrotransformation. DNA diagnosis of microorganisms. RNA diagnosis of microorganisms. Amplification techniques in molecular diagnosis. Identification of bacterial antibiotic resistence genes. Identification of genetically modificated microorganisms. Sequence analysis of some bacterial species. Reversed and classical genetics. Genetic analysis of bacteria, bacteriophages and plasmids. Use of transposons in genetic analysis. Use of genetic techniques in clinical diagnosis. Quantification of antimicrobial activity of compounds. Validation of laboratory microbiological methods.

Literature

Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.

Assessment methods (in Czech)

Přednáška je ukončena zkouškou.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.