B7430 Úvod do molekulární biotechnologie

Faculty of Science
Autumn 2001
Extent and Intensity
2/0. 2 credit(s). Type of Completion: graded credit.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(B4020) a Obecná mikrobiologie (B4090).
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO). Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
Literature
  • 1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.
Assessment methods (in Czech)
Předmět je ukončen klasifikovaným zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every week.
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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