Bi8540 Mutagenesis

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Kuglík, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Prerequisites
( Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics ) && ! B8540 Mutagenesis
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Mutagenesis envolves basic lectures from molecular biology and genetics. The main goal of the discipline is to give to students a satisfactory deep uderstanding of the molecular mechanisms of spontaneous and induced mutagenesis. The subjects of this discipline also are the environmental genotoxicants, mutagenicity tests and strategies and methods for genetic risk assessment.
Syllabus
  • 1. History of mutagenesis, types of mutations. 2. Origin of spontaneous mutation. Tautomerization, deamination of DNA bases. Role of DNA repair, replication, and recombination. 3. Induced mutations. Radiation-induced mutations. Biological effects of UV radiation. Consequences of thinning of ozone layer. 4. Biological effects of ionizing radiation. Sources and types of ionizing radiation. Radiation-induced DNA damage. Factor controlling biological effects of radiation. Biological effects of magnetic field. 5. Chemical mutagenesis and chemical mutagens. Classification of chemical mutagens. 6. Promutagens. Metabolic activation of promutagens, plant promutagens, animal promutagens. Pharmacological polymorphisms. 7. DNA damage and repair mechanisms (fotoreactivation, excision repair, postreplication repair, SOS repair). Error prone and error free DNA repair, adaptive repair. 8. Molecular basis of mutagenesis induced by physical and chemical mutagens. 9. Antimutagens. Classification of antimutagenic compound. Mechanisms of antimutagenenesis. 10. Environmental mutagens and carcinogens. 11. Method for genetic risk assessment. Identification of mutagens among potentially hazardous chemicals. 12. Monitoring environmental genotoxicant. 13. Principles of mutation tests.
Literature
  • Environmental mutagenesis. Edited by David H. Phillips - Stanley Venitt. 1st pub. Oxford: Bios scientific publishers, 1995, xix, 403 s. ISBN 1-872748-19-8. info
  • BRUSICK, David J. Methods for genetic risk assessment. Boca Raton: Lewis Publishers, 1994, 250 s. ISBN 1-56670-039-6. info
  • COOPER, David N. and Michael KRAWCZAK. Human gene mutation. Oxford: Bios scientific publishers, 1993, 402 s. ISBN 1-872748-41-4. info
Assessment methods (in Czech)
Výuka: přednáška. Zkouška: ústně.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/8540.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007.
  • Enrolment Statistics (Autumn 2003, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2003/Bi8540