PřF:C8857 Study of interactions between

C8857 Study of interactions between proteins and DNA

Faculty of Science
Autumn 2007

Extent and Intensity

1/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Mgr. Lumír Krejčí, Ph.D. (lecturer)

Guaranteed by

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Biochemistry (Eng.) (programme PřF, N-BCH)
Biochemistry (Eng.) (programme PřF, N-BCH3)
Biochemistry (programme PřF, N-BCH)
Biochemistry (programme PřF, N-BCH3)
Biochemistry (programme PřF, N-CH)
Biology (programme PřF, N-BI)
Biomolecular Chemistry (Eng.) (programme PřF, N-BCH)
Biomolecular Chemistry (Eng.) (programme PřF, N-BCH3)
Biomolecular Chemistry (programme PřF, N-BCH)
Biomolecular Chemistry (programme PřF, N-BCH3)
Macromolecular Chemistry (programme PřF, N-CH)
Macromolecular Chemistry (programme PřF, N-CH3)
Molecular Biology and Genetics (programme PřF, N-BI)
Molecular Biology and Genetics (programme PřF, N-BI, specialization Genomika a proteomika)
Molecular Biology and Genetics (programme PřF, N-BI, specialization Molekularni a geneticka diagnostika)

Syllabus

1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II

Language of instruction

English

Further Comments

Study Materials
The course is taught annually.
The course is taught every week.