PřF:Bi8858c Biocatalysis-Practice - Course Information

Bi8858c Protein Preparation and Characterization II - practice

Extent and Intensity

0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.

Timetable

Tue 13:00–14:50 C04/218

Prerequisites

NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10

fields of study / plans the course is directly associated with

• Analytical Biochemistry (Eng.) (programme PřF, N-BCH)
• Analytical Biochemistry (programme PřF, N-BCH)
• Biochemistry (Eng.) (programme PřF, N-BCH)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Biochemistry (programme PřF, N-CH)
• Biology (programme PřF, N-BI)
• Biomolecular Chemistry (Eng.) (programme PřF, N-BCH)
• Biomolecular Chemistry (programme PřF, N-BCH)
• Genomics and Proteomics (programme PřF, D-BI4)
• Microbiology (programme PřF, D-BI4)
• Molecular and Cellular Biology (programme PřF, D-BI4)
• Molecular Biology and Genetics (programme PřF, N-BI)
• General and Molecular Genetics (programme PřF, D-BI4)
• General Biology (programme PřF, N-BI)
• General Biology (programme PřF, N-BI, specialization Ekotoxikologie)
• General Biology (programme PřF, N-BI, specialization Plant Physiology)
• General Biology (programme PřF, N-BI, specialization Physiology of Animals)
• General Biology (programme PřF, N-BI, specialization Microbiology)
• Organic Chemistry (programme PřF, N-CH)

Course objectives

The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.

Syllabus

• 1. Protein folding and stability
• 2. Quarternary structure of proteins
• 3. Enzyme activity and kinetics

Teaching methods

laboratory practicals, protocols prepration, homeworks

Assessment methods

the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered (the classification is dependent on the report quality and the correctness of results)

Language of instruction

English

Further Comments

The course is taught annually.

• Enrolment Statistics (Autumn 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2009/Bi8858c