Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2010
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Timetable
Tue 13:00–14:50 C13/209
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation.
  • Enrolment Statistics (Autumn 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2010/Bi8858c