Bi7330 Cytology and morphology of bacteria

Faculty of Science
Autumn 2012
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Andrea Vávrová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Ludmila Tvrzová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 13:00–13:50 Bpt,01013
Prerequisites
general microbiology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain morphology of bacterial cells and colonies with definite stages which differ in appearance during their life-cycles; to characterize of cytologial structures and structural aspects including bacterial cytoskeleton; to understand the simple and complex life-cycles; to understand modelling of growth paterns in bacterial colonies and bacterial cell motility; to characterize of biofilm and its signal procesess and interactions between bacterial cells; to understand functional and physiological properties and adaptations on the macromolecular level structural differences between prokaryotes and eukaryotes; to work with information on the methods of the study and demonstartion of morphology of bacteria and cell structures.
Syllabus
  • Microscopic techniques, light, fluorescent and electron microscopy. Phase contrast and Nomarski contrast.
  • Microphotography, digital photography, video and digital cameras, image acquisition.
  • Image processing and analysis software, imaging techniques.
  • The structural concept of the bacterial cell. Determination of cell shape. The cytosol. Cell wall of bacteria. Structural and biochemical features of cell walls. Teichoic and teichuronic acids. Lipids and waxes, proteis. Polysaccharides. Peptidoglycan: Molecular composition, structure, and implications for taxonomy. The outer membrane. Acidoresistant bacteria. Capsules, sheets and slimes of bacteria. Inclusion bodies. Bacterial ribosome, nucleoid, cytoplasmatic membrane. Ultrastructural aspects.
  • Modelling of growth paterns in bacterial colonies
  • Structural and functional aspects of bacterial motility. Flagella. Pili and fimbriae.
  • Growth cycles of bacteria.
  • Resting stages of bacteria. Endospores, exospores, cysts.
  • Dimorphic prosthecate bacteria. Chlamydiae.
  • Complex growth cycles. Actinomycetes and myxobacteria.
  • Cyanobacteria.
  • The role of molecular composition of bacterial cell in classification and identification of bacteria.
Literature
  • PERRY, Jerome J. and James T. STALEY. Microbiology :dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0-03-053893-9. info
Teaching methods
theoretical preparing for laboratory work in practical course of Cytology and Morphology of bacteria using illustrative schemes and image documentation
Assessment methods
lectures, colloquium
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
http://www.sci.muni.cz/mikrob/cytologieosn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2025.
  • Enrolment Statistics (Autumn 2012, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2012/Bi7330