C6230 Clinical Biochemistry - laboratory course

Faculty of Science
Autumn 2013
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Michaela Wimmerová, Ph.D. (lecturer)
Mgr. Petra Sýkorová, Ph.D. (seminar tutor)
Mgr. Jana Šístková, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Michaela Wimmerová, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Michaela Wimmerová, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C6230/01: Tue 9:00–12:50 C15/212, J. Komárek
C6230/02: Tue 13:00–16:50 C15/212
Prerequisites (in Czech)
NOW( C6220 Clinical Biochemistry ) && SOUHLAS
Předpokladem zápisu je současné absolvování předmětu klinická biochemie (C6230). K zápisu je potřeba požádat o výjimku-souhlas učitele. Souhlas bude udělován v pořadí: Aplikovaná biochemie (předmět je povinný), Biochemie (předmět je povinně volitelný), ostatní.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 50 student(s).
Current registration and enrolment status: enrolled: 0/50, only registered: 0/50, only registered with preference (fields directly associated with the programme): 0/50
fields of study / plans the course is directly associated with
Course objectives
The laboratory course is designed to introduce the student to the principles and practices of clinical chemistry. Through laboratory experiences the student will develop the necessary skills to perform analytical procedures and evaluate laboratory data. The exercises familiarize the student with the performance of simple laboratory tests common in health care centers. Advanced part is focused to some special methods in laboratory diagnostics.

At the end of the course students should be able to:
understand basic principles of basic analytical parameters determination in blood serum and urine;
determine basic biochemical parameters in serum and urine;
interpret measured results in context of potential health/disease
Syllabus
  • Basic course
  • Chemical examination of urine
  • Glucose, hemoglobin
  • Alkaline phosphatase, lipids, cholesterol
  • Nonprotein nitrogenous compounds in serum
  • Liver biochemistry; enzymes, bilirubin
  • Different approaches in isoenzyme diagnostics: creatine kinase and lactate dehydrogenase models
  • Advanced course
  • HPLC - assay of ascorbic acid
  • LC-MS analysis and identification of a BSA proteolytic digest
  • Enzyme biosensors in clinical diagnostics
  • Uric acid assay using capillary zone electrophoresis
  • Immunochemistry-ELISA
  • Excursion to hospital and private laboratory of clinical biochemistry
Literature
  • RACEK, Jaroslav. Klinická biochemie. 2., přeprac. vyd. Praha: Galén, 2006, 329 s. ISBN 8072623249. info
  • Klinická biochemie :požadování a hodnocení biochemických vyšetření. Edited by Jaroslav Masopust. 1. vyd. Praha: Karolinum, 1998, 429 s. ISBN 80-7184-648-1. info
Teaching methods
home preparation, laboratory courses
Assessment methods
class discussion, written protocols, credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://orion.chemi.muni.cz/michaw/teaching.htm
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2013, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2013/C6230