C5995 C5995 Bioanalytical Methods

Faculty of Science
Autumn 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should understand this topics - basic biochemical separation and analytical methods. Principles, instrumentation, and use. Oriented mainly towards routine applications.
Syllabus
  • 1. Introduction. Objects of biochemical study, their characteristics and specific properties. Basic principles of treatment.
  • 2. Analytical aspects of separation methods. Chromatography.
  • 3. Mass spectroscopy. Types, instrumentation, application.
  • 4. Spectral methods. Atomic spectroscopy.
  • 5. UV-Vis photometry. Principles, limitations, instrumentation.
  • 6. Fluorimetry, application in biochemistry. Instruments, limitation.
  • 7. Disperse methods, chiral environment.
  • 8. Electromigration methods, types. Electrophoresis, types, matrices, practice.
  • 9. Electrochemical methods. Potentiometry and amperometry.
  • 10. Ion selective electrodes, types, measurement, application in biochemistry.
  • 11. Amperometry, polarography and voltammetry. Biochemical application.
  • 12. Immunochemical methods, representatives, application.
  • 13. Other methods. Osmometry.
  • 14. Principles of statistical evaluation.
Literature
  • Ferenčík M., Škárka B.: Biochemické laboratórne metódy, Alfa Bratislava 1981
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986, 199 s. info
  • KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987, 430 s. URL info
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2., přeprac. a rozš. vyd. Praha: Academia, 2000, 337 s. ISBN 8020007911. info
  • Králová Blanka: Bioanalytické metody, VŠChT Praha 2001
Teaching methods
Lectures.
Assessment methods
Lecture. Oral examination.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013.
  • Enrolment Statistics (recent)
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