C5855 Methods in Biophysical Chemistry

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Libuše Trnková, CSc. (lecturer)
doc. RNDr. Mgr. Jozef Hritz, Ph.D. (lecturer)
doc. Mgr. Zdeněk Farka, Ph.D. (lecturer)
doc. Mgr. Pavel Plevka, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. Veronika Štěpánková, Ph.D. (lecturer)
doc. Mgr. David Bednář, Ph.D. (lecturer)
RNDr. Mgr. Iveta Třísková, Ph.D. (lecturer)
doc. Mgr. Radka Chaloupková, Ph.D. (lecturer)
prof. Dr. Jiří Kozelka, PhD. (lecturer)
Guaranteed by
prof. RNDr. Libuše Trnková, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Mon 14:00–15:50 C12/311
Prerequisites
exam of C5850 Introduction to Biophysical Chemistry
simultaneous attendance of C5855 Methods in Biophysical Chemistry is recommended
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students of this course will gain extensive physicochemical foundations in genomics, proteomics, biotechnology and bionanotechnology. Students will learn in detail with modern approaches in biophysically-chemical research, very useful in biophysics, biochemistry, medicine, pharmacy and food industry. At the end of this course the student will be able: a) to understand the principles of biophysical methods, b) to explain the principles of modern biophysical methods, c) to use the fundamentals of physical chemistry in biological practice d) to use the knowledge gained for the preparation of the thesis, e) to use the knowledge gained in theory (structure calculation) and experience (experiment to verify the theoretical calculations) f) to interpret new trends, tactics and strategies of biophysical chemistry.
Learning outcomes
At the end of this course the student will be able: a) to understand the principles of biophysical methods, b) to explain the principles of modern biophysical methods, c) to use the fundamentals of physical chemistry in biological practice d) to use the knowledge gained for the preparation of the thesis, e) to use the knowledge gained in theory (structure calculation) and experience (experiment to verify the theoretical calculations) f) to interpret new trends, tactics and strategies of biophysical chemistry. Na konci tohoto kurzu bude student schopen: a) porozumět principům biofyzikálních metod, b) vysvětlit moderní principy biofyzikálních metod, c) použít základy fyzikální chemie v biologické praxi, d) nabyté vědomosti použít v přípravě bakalářské práce, e) získané vědomosti využít v teorii (výpočty struktur) a v praxi (experiment, který teoretické výpočty ověří), f) interpretovat nové trendy, taktiky a stratégie biofyzikální chemie.
Syllabus
  • 1. The behavior of biological macromolecules (nucleic acids, proteins and polysaccharides)
  • 2. Biophysical chemistry cells and membranes (physical chemistry in biology and biophysics of cells and membranes, membrane transport)
  • 3. Techniques for studying the structure and function of biopolymers (method's distribution, advantages and disadvantages, applications, general procedure for solving the structure of biopolymers using different techniques)
  • 4. Introduction to Fourier methods and statistics (the use of Fourier transforms in intensive experimental methods, the advantage of a mathematical signal processing)
  • 5. Biological methods for the analysis of primary, secondary and tertiary structure of biomolecules (chemical sequencing method, PCR)
  • 6. Physical methods for the analysis of primary, secondary and tertiary structures of biomolecules (X-ray analysis, electron microscopy, light scattering)
  • 7. Spectral methods for analyzing the primary, secondary and tertiary structure of biomolecules (UV-Vis, CD, NMR, MS)
  • 8. Electrochemical methods for the analysis of primary, secondary and tertiary structures of biomolecules (electrophoresis, dynamic methods, biosensors)
  • 9. Bioinformatics, genomics and proteomics (databases, sequence analysis, genome and metagenome, microarray technology, protein-protein and protein-DNA interactions)
  • 10. Experimental methods in molecular Biotechnology I. and II. (isolation, amplification, sequencing, and recombinant DNA, expression and isolation of proteins, methods of structural and functional characterization of proteins)
  • 11. Molecular biotechnology in medicine, environmental protection, industry and agriculture (cell and gene therapy, tissue engineering and regenerative medicine, molecular diagnostics, vaccines, biosensors, bioremediation, biofuels, environmental protection and sustainable development, the use of biomass, fermentation technology, biocatalysis in the pharmaceutical and chemical industries, gray and white biotechnology in the food industry, genetic engineering of plants and animals, cloning, GMO foods, biopesticides)
  • 12. Computer modeling of the structures of nucleic acids and proteins (structural and functional significance of DNA, RNA, proteins, methods of prediction of biopolymer secondary structures).
Literature
    required literature
  • ALLEN, James P. Biophysical chemistry. Oxford: Wiley-Blackwell Pub., 2008, xvi, 492. ISBN 9781405124362. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part II, Techniques for the study of biological structure and function. 12th print. New York: W.H. Freeman and Company, 2001, xxix, s. 3. ISBN 0-7167-1189-3. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part I, The conformation of biological macromolecules. 11th print. New York: W.H. Freeman and Company, 1999, xxii, 341. ISBN 0-7167-1042-0. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part III, The behavior of biological macromolecules. New York: W.H. Freeman and Company, 1980, xxix, s. 8. ISBN 0-7167-1191-5. info
    recommended literature
  • Biophysical chemistry of biointerfaces. Edited by Hiroyuki Ohshima. Hoboken: Wiley, 2010, xvi, 547 p. ISBN 9780470169353. info
Teaching methods
lectures, tests, discussions, ppt presentations
Assessment methods
written test and oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2008, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2018, recent)
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