PřF:C9035 Evol. plant cytogenet. practic - Course Information
C9035 Evolutionary and comparative plant cytogenetics - practice
Faculty of ScienceAutumn 2018
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- prof. Mgr. Martin Lysák, Ph.D., DSc. (seminar tutor)
RNDr. Terezie Malík Mandáková, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. Mgr. Martin Lysák, Ph.D., DSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science - Prerequisites
- Prerequisites: basic knowledge in the field of cytogenetica and cytology (e.g., pipetting, preparation of solutions, microscopy).
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- The participant will master advanced techniques of plant cytogenetics (microscopic preps, advanced fluorescence microscopy, chromosome painting, genomic in situ hybridization, image processing).
- Learning outcomes
- Student will be able to:
- prepare micrscopic preparations;
- label isolated DNA by haptens and fluorochromes;
- prepare DNA probes;
- carry out fluorescence in situ hybridization (FISH) of the labeled DNA probes to chromosomes and cell nuclei;
- analyze preparations using fluorescence microscopy;
- photograph chromosomes and cell nuclei;
- analyze and process the acquired photographs. - Syllabus
- Introduction to evolutionary and comparative plant cytogenetics. Principles of light and fluorescence microscopy. Preparation of mitotic and meiotic chromosomes from roo tip meristems and anthers, microscopic analysis of chromosome preparations. Direct and indirect labelling of DNA probes by nick translation. Fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), comparative chromosome painting (CCP). Immunodetection indirectly labelled DNA probes. Fluorescence microscopy of chromosome preps after FISH, GISH a CCP. Processing of microscopy photographs (Adobe Photoshop), interpretations.
- Literature
- recommended literature
- MANDÁKOVÁ, Terezie and Martin LYSÁK. Chromosome Preparation for Cytogenetic Analyses in Arabidopsis. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 1, No 1, p. 43-51. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20009. URL info
- MANDÁKOVÁ, Terezie and Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 4, No 2, p. 359-371. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20022. URL info
- SCHWARZACHER, T. and HESLOP-HARRISON. Practical in situ hybridization. Bios, 2000. info
- Teaching methods
- Practical tasks in a cytogenetic laboratory.
- Assessment methods
- Colloquium.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Note related to how often the course is taught: blok 5 dni.
General note: Výuka proběhne ve dnech 4.-8.11.2013.
- Enrolment Statistics (Autumn 2018, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2018/C9035