F9070 Experimental methods of biophysics

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: zk (examination).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
doc. Mgr. Pavel Plevka, Ph.D. (lecturer)
Mgr. Daniel Renčiuk, Ph.D. (lecturer)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Tue 15:00–16:50 C02/211
Prerequisites
Basic methods of physical chemistry and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The objective of the lectures, practical demonstrations and excursions is to acquaint with range of methods that enable us to quantitatively and qualitatively describe interactions of proteins, DNA and small molecules such as drugs on one hand and methods that describe behavior of cells and whole organisms on the other hand. We will learn
- describe and explain principles of methods for study of interactions, structure and function of biomolecules
- apply the methods and approaches to practical case studies
- use and broaden our knowledge during research and development laboratories of companies e.g. TESCAN, BIOVENDOR and FEI - ThermoFisher Scientific
Syllabus
  • Introduction - selection of three main areas of lectures based on individual students' request
  • Separation methods (HPLC)
  • Electrophoresis of biopolymers
  • Flowcytometry
  • Vibrational spectroscopy (IR, Raman, ROA)
  • Factor analysis in spectroscopy
  • CD and LD spectroscopy
  • Calorimetry of biopolymers
  • Scaning probe microscopy
  • Fluorescence spectroscopy (FRET)
Literature
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2. přeprac. a rozš. vyd. Praha: Academia, 2000, 337 s. ISBN 8070802731. info
  • PROSSER, Václav. Experimentální metody biofyziky. 1. vyd. Praha: Academia, 1989, 712 s. ISBN 8020000593. URL info
Teaching methods
lectures with class discussion, case studies
Assessment methods
Colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/F9070