C5855 Methods in Biophysical Chemistry

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Libuše Trnková, CSc. (lecturer)
doc. RNDr. Mgr. Jozef Hritz, Ph.D. (lecturer)
RNDr. Mgr. Iveta Třísková, Ph.D. (lecturer)
doc. Mgr. Zdeněk Farka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Libuše Trnková, CSc.
Prerequisites
exam of C5850 Introduction to Biophysical Chemistry
simultaneous attendance of C5855 Methods in Biophysical Chemistry is recommended
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students of this course will gain physicochemical foundations of biophysical methods used in genomics, proteomics, biotechnology and bionanotechnology.
Learning outcomes
Students will learn in detail with modern approaches in biophysically-chemical research, very useful in biophysics, biochemistry, medicine, pharmacy and food industry. At the end of this course the student will be able:
a) to explain the principles of biophysical methods,
b) to use the fundamentals of physical chemistry in biological practice,
c) to use the knowledge gained for the preparation of the bachelor thesis,
d) to use the knowledge gained in theory (structure calculation) and experience (experiment to verify the theoretical calculations),
e) to interpret new trends, tactics and strategies of biophysical chemistry.
Syllabus
  • 1. Experiment of biophysical chemistry (tactics and strategy of choice, general procedure for solving structures and interactions of biomolecules)
  • 2. Transport in biological system (diffusion, osmosis, viscosity)
  • 3. Techniques of study of biomolecular transport (centrifugation, chromatography, electrophoresis)
  • 4. Electrical potential, energy, heat (electrophysiology, mass spectrometry, calorimetry)
  • 5. Scattering, refraction and interpretation of their data
  • 6. Electron and vibrational spectroscopy I (energy states, UV-Vis, infrared and Raman spectroscopy)
  • 7. Electron and vibrational spectroscopy II (fluorescence, FRET, phosphorescence, chiroptic spectra, X-ray spectroscopy)
  • 8. Magnetic resonance (nuclear magnetic resonance - NMR and electron paramagnetic resonance - EPR)
  • 9. Microscopy (electron microscopy - SEM and TEM, atomic force microscopy - AFM)
  • 10. Methods of study of own possibilities (manipulation, electrical and optical properties of biomolecules)
  • 11. Electrochemical methods and their use in biosensors (bioelectrochemistry, nanoelectrochemistry)
  • 12. Mathematical and statistical data processing (introduction to Fourier methods, multicomponent analysis, artificial neural networks).
Literature
    required literature
  • CAMPBELL, Iain D. Biophysical techniques. Oxford: Oxford University Press, 2012, ix, 353. ISBN 9780199642144. info
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. Vyd. 3., opr. a rozš. Praha: Academia, 2013, 423 s. ISBN 9788020022417. info
    recommended literature
  • ALLEN, James P. Biophysical chemistry. Oxford: Wiley-Blackwell Pub., 2008, xvi, 492. ISBN 9781405124362. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part II, Techniques for the study of biological structure and function. 12th print. New York: W.H. Freeman and Company, 2001, xxix, s. 3. ISBN 0-7167-1189-3. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part III, The behavior of biological macromolecules. New York: W.H. Freeman and Company, 1980, xxix, s. 8. ISBN 0-7167-1191-5. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part I, The conformation of biological macromolecules. 11th print. New York: W.H. Freeman and Company, 1999, xxii, 341. ISBN 0-7167-1042-0. info
Teaching methods
lectures, ppt presentations, discussions
Assessment methods
written test and oral exam
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2008, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
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