PřF:C7023 Separation methods A - Informace o předmětu
C7023 Separation methods A
Přírodovědecká fakultapodzim 2022
- Rozsah
- 2/0/0. 2 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- basic knowledge of physical and analytical chemistry is prerequisited.
- Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- the main objective of the course is to acquaint students with separation methods based on equilibrium separation between two phases.
- Výstupy z učení
- at the end of the course students should be able:
to manage general principles of analytical separation and its description
to manage principles of equilibrium separation between two phases
to describe and explain basic separation modes using the following phase systems - liquid-liquid, solid-liquid, solid-gas, liquid-gas
to describe and explain principles and practical aspects of liquid and gas chromatography. - Osnova
- 1. introduction to separation: separation of two ideally miscible compounds, separation limits, separation methods classification – preparative, analytical, basic principles of separation – molecular equilibrium, intermolecular interactions, entropy.
- 2. extraction: description of extraction equilibria in system liquid-liquid and in system liquid-solid; choice of extraction system, carry-out of extraction, influence of analyte-solvent interaction, repeated extraction
- 3. preanalytical sample preparation: CCE (counter-current extraction), SFE (supercritical fluid extraction); ASE (accelerated solvent extraction); MAE (microwave assisted extraction), SPE (solid phase extraction); SPME (solid phase microextraction), HSE (head-space extraction)
- 4. analytical separation: chromatography, basic techniques (frontal, displacement, elution), theoretical principles – column equilibrium, physico-chemical description of processes on LC, kinetic and thermodynamic aspects of separation.
- 5. study and description of separation; quantitative relations between structure and selectivity in regard to retention; liquid chromatography (LC) – history, theoretical ground; classification of basic separation mechanisms in LC (LLC, LSC, IEC), LC arrangement: mobile phase delivery, elution and elution force (isocratic and gradient); injection devices; separation column, types of stationary phases (particle, monolithic, on-chip), overview of sorbents;
- 6. detectors photometric (DAD), refractometric, fluorescence, amperometric, conductivity, light-scattering, mass spectrometric, derivatisation and sample collection. description and evaluation of separation results: defining the chromatographic system, analytical information in chromatogram (qualitative, quantitative); precision, accuracy, limits of analysis (MDL, LOD, LOQ); evaluation of separation efficiency, testing of the system and its “good” behaviour;
- 7. basic LC modes: CCC (counter-current chromatography), NP-HPLC (normal phase liquid chromatography), RP-HPLC (reverse phase liquid chromatography), TLC (thin-layer chromatography, HPTLC 2D TLC), ultra-high performance chromatography, high temperature liquid chromatography, elevated-temperature ultra-high performance chromatography, hydrophilic interaction chromatography (HILIC), ERLIC, hydrophobic interaction chromatography (HIC),
- 8. IEC (ion exchange chromatography) – principles and carry-out, IXC (ion exclusion chromatography), chromatofocusation; affinity chromatography – principles, IMAC (immobilised-metal affinity chromatography); SFC (supercritical fluid chromatography); PC (perfusion chromatography);
- 9. multi-dimensional chromatography: interface and principles; hyphenated separation techniques; preparative chromatography: methods (system scale-up, system overloading), fraction collection,
- 10. gas chromatography (GC): history, differences between LC and GC (isotherms, gas compressibility, retention quantities), GC arrangement, mobile phase delivery; injection device (split, splitless); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), column thermostat, detectors (flame ionisation, chemiluminescence, mass spectrometric detectors, etc.), GC system definition, GC evaluation and testing.
- Literatura
- MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3. vyd. Chichester: J. Wiley & Sons, 1999, 338 s. ISBN 0-471-98372-1. info
- A practical approach to chiral separations by liquid chromatography. Edited by Ganapathy Subramanian. Weinheim: VCH Verlagsgesellschaft, 1994, 405 s. ISBN 3-527-28288-2. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentations themselves will be available in both, czech & english, as study materials (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation, and because there are no available textbooks in czech language covering certain parts of the subject.
the subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise, students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial at max 2x2h per semester; contact the lecturer to set up the schedule. - Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. the examination consists of three principal questions.
- Vyučovací jazyk
- Angličtina
- Navazující předměty
- Informace učitele
- the lecture is going to be merged with subject C7021 and taught in the same time slot - every tuesday 11:00-12:50 in C14-207
- Další komentáře
- Studijní materiály
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Výuka probíhá každý týden. - Nachází se v prerekvizitách jiných předmětů
- Statistika zápisu (podzim 2022, nejnovější)
- Permalink: https://is.muni.cz/predmet/sci/podzim2022/C7023