F9070 Experimental methods of biophysics

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
doc. Mgr. Karel Kubíček, PhD. (lecturer)
Mgr. Jan Přibyl, Ph.D. (lecturer)
doc. Mgr. Karel Souček, Ph.D. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Karel Kubíček, PhD.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: doc. Mgr. Karel Kubíček, PhD.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Timetable
Tue 15:00–16:50 C02/211
Prerequisites
Basic methods of analytical chemistry and biophysics and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of this course is to provide students with the ability to
- list and describe techniques necessary for study of structure and function of biomolecules
- apply this knowledge to DNA and proteins study.
Learning outcomes
Students will be able to:
- understand principles of biophysical methods of experimental analyses of function and structure DNA and proteins
- apply and combine biophysical methods for investigation of interactions and functions of biomolecules
Syllabus
  • Introduction
  • Separation methods (HPLC)
  • Electrophoresis of biopolymers
  • Flowcytometry
  • Vibrational spectroscopy (IR, Raman, ROA)
  • Factor analysis in spectroscopy
  • CD and LD spectroscopy
  • Calorimetry of biopolymers
  • Scaning probe microscopy
  • Fluorescence spectroscopy (FRET)
Literature
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2. přeprac. a rozš. vyd. Praha: Academia, 2000, 337 s. ISBN 8070802731. info
  • PROSSER, Václav. Experimentální metody biofyziky. 1. vyd. Praha: Academia, 1989, 712 s. ISBN 8020000593. URL info
Teaching methods
lectures with class discussion
Assessment methods
Colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/F9070