Bi6700c Taxonomy of prok.-lab.course

Faculty of Science
Autumn 2024
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Dana Nováková, Ph.D. (seminar tutor)
prof. RNDr. Ivo Sedláček, CSc. (seminar tutor)
doc. RNDr. Pavel Švec, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Ivo Sedláček, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi6700 Taxonomy of prokaryotes )
Examination - basic microbiology, physiology of bacteria, biochemistry, molecular biology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main goal of this course is introduction of students in isolation and cultivation of microorganisms and maintenance of pure cultures. The next step is the microscopic and macroscopic morphology of archaea and bacteria, their phenotypic classification and identification and typing of bacteria by rep-PCR method as well as by mass spectrometry MALDI TOF procedure.
Learning outcomes
At the end of this course students should be able to perform: - isolation and cultivation of microorganisms, maintenance of pure cultures; - microscopic and macroscopic morphology of archaea and bacteria; - phenotypic classification and identification of Gram negative bacteria; - phenotypic classification and identification of Gram positive bacteria; - typing and identification of bacteria by rep-PCR method; - analysis of microorganisms by mass spectrometry MALDI TOF procedure.
Syllabus
  • Agar and broth media
  • Isolation
  • Cultivation
  • Morphology
  • Classification of fermenting rods
  • Classification of non-fermenting rods
  • Classification of G+ k cocci and rods (phylum Actinobacteria, Firmicutes)
  • rep_PCR
  • MALDI TOF MS
  • Evaluation of identification
Literature
    required literature
  • SEDLÁČEK, Ivo. Taxonomie prokaryot (Taxonomy of prokaryotes). 1st ed. Brno: Masarykova univerzita, 2007, 270 pp. 55-969C-2006 02/58 12Př. ISBN 80-210-4207-9. info
    recommended literature
  • Murray, PB., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H. (eds.). 2003. Manual of clinical microbioloogy. Volume 1. 8th edition. ASM Press. Washington, D.C.
  • Pershing, D.H., Tenover, F.C., Versalovic, J., Tang, Y.W., Unger, E.R., Relman, D.A., White, TJ. (eds.). 2004. Molecular microbiology: diagnostic principles and practice. ASM Press, Washington, USA.
Teaching methods
Individual study followed by laboratory practice.
Assessment methods
Two written tests before course (30 and 50 questions), lacoratory courses, credit (course).
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Limitujici je kapacita cvicebny
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/Bi6700c