C5700 Biochemistry

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. Mgr. Tomáš Kašparovský, Ph.D. (lecturer)
Mgr. Lukáš Faltinek (seminar tutor)
Guaranteed by
prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 18:00–19:50 B11/335
Prerequisites
!NOWANY( C3580 Biochemistry )
Organic Chemistry I, II or Backgrounds of Organic Chemistry. Physical Chemistry I, II or Backgrounds of Physical Chemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand and explain - Structure of Living Matter. Composition and Organization of Cells. Basic Classes of Biomolecules: Amino Acids and Proteins, Sugars and Polysaccharides, Nucleic Acids, Lipids and Membranes. General Features of Metabolism. Energetics of Biochemical Reactions. Introduction to Enzymes and Coenzymes. Metabolic Fate of Biomolecules. Metabolism of Amino Acids and Proteins. Metabolism of Nucleic Acids and Proteosynthesis. Metabolism of Saccharides and Lipids. Basic Bioenergetic Metabolism. Integration of Metabolism, Regulation Principles. Membrane Transport. Special Biosynthetic Pathways.
Learning outcomes
After completing the course, student will have deep knowledge and understanding of basic biochemical processes. He will be qualified to explain biochemical curriculum to the high school students.
Syllabus
  • 1. Introduction. Composition of living matter, hierarchy of structures. Biogenic elements. Important functional groups.
  • 2. Amino acids, their properties. Proteinogenic amino acids, free amino acids. Peptides. Sequence and its determination. Biochemically important peptides. Protein structure, conformation and its changes. Methods of studying proteins.
  • 3. Carbohydrates. General properties, distribution of monosaccharides. Derivatives. Free monosaccharides. Glycosides, di-, oligo- and polysaccharides. Distribution, structure and function in the organism. Glycoproteins and proteoglycans.
  • 4. Lipids. Composition, properties, distribution and function. Practical aspects - fats.
  • 5. Nucleic acids. Composition and structure of DNA and RNA, properties, study methods.
  • 6. Enzymes, their structure, properties, distribution and nomenclature. Speed ​​of enzyme reactions, activity, conditions. Enzyme kinetics.
  • 7. Glycolysis, reaction scheme, energetics, regulation. Gluconeogenesis. Other carbohydrate conversions. Pentose phosphate pathway.
  • 8. Cycle of tricarboxylic acids. Reaction scheme, energetics. Biosynthetic significance, regulation. Protein catabolism. Digestion. General amino acid conversions. nitrogen metabolism.
  • 9. Lipid metabolism. Degradation and biosynthesis of fats and phosphatides. Degradation and biosynthesis of fatty acids. Ketone substances.
  • 10. Oxidative phosphorylation. Organization of the respiratory chain, protonmotive force, generation of ATP. Photosynthesis, pigments, electron transfer and the formation of ATP. CO2 fixation.
  • 11. Structure and meaning of biomembranes. Compartmentation. membrane transport.
  • 12. Regulation of metabolism. Mechanism of action of hormones.
Literature
  • KODÍČEK, Milan, Olga VALENTOVÁ and Radovan HYNEK. Biochemie : chemický pohled na biologický svět. 3. přepracované vydání. Praha: Vysoká škola chemicko-technologická, 2022, 395 stran. ISBN 9788075921246. info
  • NELSON, David L. and Michael M. COX. Lehninger principles of biochemistry. 5th ed. New York: W.H. Freeman and Company, 2008, xxix, 1158. ISBN 9780716771081. info
  • DOSTÁL, Jiří, Hana PAULOVÁ, Jiří SLANINA and Eva TÁBORSKÁ. Biochemie pro posluchače bakalářských oborů. 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2017, 158 pp. ISBN 978-80-210-5020-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • MURRAY, Robert K. Harperova biochemie. Vyd. v ČR 4., V H & H 3. Jinočany: H & H, 2002, ix, 872. ISBN 8073190133. info
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, xiv, 1325. ISBN 8085605449. info
Teaching methods
Theoretical lecture.
Assessment methods
Lecture. Oral examination.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
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