E0311 Chromatographic Methods

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 11:00–12:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- define fundamentals of chromatographic methods and describe them in the context with other separation analytical methods;
- understand and clarify principles of individual types of chromatography and functional layout of chromatographic instrumentation, their individual parts and detecting elements;
- explain theoretical aspects and mechanisms of chromatographic separation and principles of choice of chromatographic systems;
- asses and interpret results of chromatographic analyses;
- evaluate significance of connection of chromatographic methods with various analytical techniques, especially with spectral methods;
- take advantage of chromatography in quantitative and qualitative analyses of various types of samples;
Syllabus
  • 1. Chromatographic separation, retention parameters, chromatogram, measure of separation, separation efficiency. chromatographic zones.van Deemter equation a Golay equation. Retention indexes.
  • 2. Gas chromatography.
  • 3. Gas-liquid chromatography. Columns, liquid phases, carriers.
  • 4. Adsorption gas chromatography. Characteristics , comparison with GLC. Adsorbents, application.
  • 5. Capilary column, WCOT, PLOT, SCOT. Quality evaluation.
  • 6. Mobile phases, gas mobile phases comparison.
  • 7. GC instrumentation, sample injection, detection.
  • 8. Liquid chromatography. Stationary and mobile phases, separation properties
  • 9. Column, stationary phases, sorbents properties.
  • 10. Mobile phases, classification of solvents, elution techniques.
  • 11. Techniques, principles, retention models, separation strategy, application.
  • 12. Instrumentation, pumps, injection devices, detectors and detection principles.
  • 13. Trends in development of chromatographic methods.
  • 14. Examples of chromatographic applications.
Literature
  • POOLE, C. F. The essence of chromatography. 1st ed. Amsterdam: Elsevier, 2003, ix, 925. ISBN 0444501991. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3rd ed. Chichester: J. Wiley & Sons, 1999, 338 pp. ISBN 0-471-98372-1. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
Teaching methods
Teaching is conducted in the form of self-study of study materials in English and individual consultations. Students will receive copies of individual images in PowerPoint and an electronic version stored in IS. The intelligibility of difficult parts is verified interactively during individual consultations.
Assessment methods
The final assessment (at the end of the semester) is by oral examination. Three related topics are the content of the discussion during the oral examination.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught each semester.
The course is also listed under the following terms Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
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