BLLC0111c Medical Chemistry - practice

Faculty of Medicine
Autumn 2008
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
PharmDr. Lenka Adámková, Ph.D. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
Mgr. Martin Klepárník, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Lenka Nerudová (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Guaranteed by
prof. MUDr. Vladimír Palyza, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: Lenka Nerudová
Timetable
Tue 13:00–15:30 A16/211
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course is to become oriented in the enviroment of chemical laboratory and its equipment, to acquire simple laboratory procedures and techniques, to practice common separation procedures and become familiar with handling common laboratory machines.Students will learn to handle laboratory data into the lab reports, to solve the problems and derive conclusions.
Syllabus
  • Rules of study and directions for laboratory work General safety guidelines. Handling chemical substances – main regulations. Measuring of liquid volume. Advantages and disadvantages of plastic ware. Clearing of glass and plastics. Heating, cooling, freezing. Thermostats. Weighing, types of weights. Demonstration: Various types of weights, handling with them. Demonstration: Volumetric glassware Demonstration: Pressure cylinders and manipulation with them Measuring of small volumes of liquids, calibration of pipettors, expression of accuracy and precision. Demonstration: using of pipettors, principles of precise pipetting Gravimetric calibration of micropipettors. Verification of precision of repeated pipettting. Preparation of solutions with given concentration. Photometric verification of concentration Filtration, dialysis, ultrafiltration and microfiltration , centrifugation. Demonstration: Using of various centrifuges, replacement of rotors, calculations. Preparation of saline solution. Purification of Al2O3 for chromatography Dialysis of starch and podium chloride. Separation of precipitated proteins by centrifugation. Distillation, extraction. Extraction of drugs from urine. Demonstration : Extraction on solid phase. Preparation of redistilled water. Demonstration: Extraction on Soxhlet apparatus Demonstration: Using of vacuum evaporator Measuring and calculation pH of solutions of acids, bases and salts, pH meters a indicator strips. Preparation of volumetric solutions. Acid-base titrations, neutralization titration curves.. Determination of NaOH concentration using volumetric solution of oxalic acid. Determination of HCl concentration. Construction of titration curves of hydrochloric and acetic acid. Solution of buffers and their preparation. Preparation of hydrogenphosphate buffer of various pH values. Titration of a buffer. Determination of bufferin kapacity. Ionic reactions. Ions in dribling water. Solubility of compounds. Ion-selective electrodes. Detection and quantitative determination of nitrates, nitrites and amonium cations in water. Properties and reactions of phosphate ions. Properties and reactions of carbonate ions. Liquid chromatography – types of, basic methods, gas chromatography. Separation of potassium dichromate and blue-dextran by gel chromatography. Adsorption chromatography on the thin layer of silica gel. Demonstration: High pressure liquid chromatography. Demonstration: Deionization of water, preparation of ultra pure water. Spectrophotometry Spektrofotometric determination of salicylic acid Simple test-tube reaction of organic compounds. Determination of ethanol in blood theory, calculations. Reduction properties of monosaccharides and L-ascorbic acid Color reactions of amino acids and proteins. Properties of proteins, quantification of proteins. Determination of isoelectric point of cassein Solubility of proteins, protein precipitation.
Literature
Assessment methods
The course is realized in laboratories. Students are doing simple experiments according to the instructions in the textbook.On the base of results they prepare the lab-reports. Conditions for giving credit:Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports and handing them to the teacher till given date.Twelve test are writen during the semester. Student will recieve course unit credit, if he has at least 70% of points. The other students will write the credit test.This test has 30 questions and student must obtain at least 12 points. The test can be once time repeated.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2008/BLLC0111c