BLKLM0321c Medical Microbiology I - practice

Faculty of Medicine
Autumn 2016
Extent and Intensity
0/0/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Jana Bednářová, Ph.D. (seminar tutor)
MUDr. Markéta Hanslianová (seminar tutor)
MUDr. Alena Ševčíková (seminar tutor)
MUDr. Ivana Vítková (seminar tutor)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Prerequisites
BLKMB011p Mol.andCell.Biol.-lect.
Basic knowledge of laboratory work expected.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Syllabus
  • 1. Safety and health protection in microbiological laboratory. Microscopy - wet mount, simple staining
  • 2. Diagnostic Gram staining
  • 3. Staining of capsulla, review
  • 4. Culure media, preparing blood agar
  • 5. Conditions of culture, blood agar culturing, growth of bacteria on culture media
  • 6. Evaluation of cultures, biochemical identification tests
  • 7. Antibiotic susceptibility testing - disc tests, MIC demostration, E-test demonstration
  • 8. Animal experiment
  • 9. Introduction to serology - reactions, serum dilution, titer counting, pipetting
  • 10. Slide agglutiation, agglutination on carriers
  • 11. Agglutination in test tubes, Widal reaction
  • 12. Serologic reactions – AKE, RRR
  • 13. Complementfixing test
  • 14. Neutralisation – ASO
  • 15. ELISA
  • (16.–30. see BLLM0322c)
Literature
    recommended literature
  • VOTAVA, Miroslav. Lékařská mikrobiologie : vyšetřovací metody. Brno: Neptun, 2010, 495 s. ISBN 9788086850047. info
    not specified
  • JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi : bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011, 73 s. ISBN 9788021056572. info
Teaching methods
practical training
Assessment methods
For credit, it is necessary:
(1) to absolve all preactical sessions, but:
- two absences, either justified of approved by a teacher are possible; even so student has to demonstrate completing the missing topics
- more absences should be substituted
- not justified absences are not tolerated
(2) complete report of all practicals
(3) succesfully written credit test, eventually also other current tests; information about all tests will be given in advance.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Podmínky zápočtu jsou uvedeny v Doplňkových údajích k předmětu
The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 5 hodin za semestr.
The course is also listed under the following terms Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2017, autumn 2018, autumn 2019.
  • Enrolment Statistics (Autumn 2016, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2016/BLKLM0321c