LF:BLKLM0321c Medical Microbiology I - pr. - Course Information

BLKLM0321c Medical Microbiology I - practice

Extent and Intensity

0/0.4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

MUDr. Jana Bednářová, Ph.D. (seminar tutor)
MUDr. Markéta Hanslianová (seminar tutor)
MUDr. Alena Ševčíková (seminar tutor)
MUDr. Ivana Vítková (seminar tutor)
Michaela Gregorovičová (assistant)

Guaranteed by

MUDr. Jana Bednářová, Ph.D.
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable of Seminar Groups

BLKLM0321c/01: Fri 26. 10. 13:00–15:30 N05079, Fri 16. 11. 13:00–14:40 ÚP P01013, J. Bednářová
BLKLM0321c/02: Fri 26. 10. 14:00–15:40 ÚP P01013, Fri 16. 11. 14:00–16:30 N05079, J. Bednářová

Prerequisites

BLKMB011p Mol.andCell.Biol.-lect.
Basic knowledge of laboratory work expected.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Laboratory Assistant (programme LF, B-SZ)

Course objectives

At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, ELISA, Western blot

Learning outcomes

At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, ELISA, Western blot

Syllabus

• 1. Safety and health protection in microbiological laboratory. Microscopy - wet mount, simple staining
• 2. Diagnostic Gram staining
• 3. Staining of capsulla, review
• 4. Culure media, preparing blood agar
• 5. Conditions of culture, blood agar culturing, growth of bacteria on culture media
• 6. Evaluation of cultures, biochemical identification tests
• 7. Antibiotic susceptibility testing - disc tests, MIC demostration, E-test demonstration
• 8. Introduction to serology - reactions, serum dilution, titer counting, pipetting
• 9. Slide agglutiation, agglutination on carriers
• 10. Agglutination in test tubes, Widal reaction
• 11. Serologic reactions – precipitation
• 12. Complementfixing test
• 13. Neutralisation – ASO
• 14. ELISA

Literature

recommended literature
• VOTAVA, Miroslav. Lékařská mikrobiologie : vyšetřovací metody. Brno: Neptun, 2010, 495 s. ISBN 9788086850047. info

not specified
• JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi : bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011, 73 s. ISBN 9788021056572. info

Teaching methods

practical training

Assessment methods

For credit, it is necessary:
to absolve all preactical sessions, but:
- one absence, either justified of approved by a teacher is possible; even so student has to demonstrate completing the missing topics
- more absences should be substituted
- not justified absences are not tolerated

Language of instruction

Czech

Follow-Up Courses

• BLKLM0422c Medical Microbiology II - practice

Further comments (probably available only in Czech)

Information on completion of the course: Podmínky zápočtu jsou uvedeny v Doplňkových údajích k předmětu
The course is taught annually.
Information on the extent and intensity of the course: 5.

• Enrolment Statistics (autumn 2018, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2018/BLKLM0321c