BVLC011c Medical Chemistry - practice

Faculty of Medicine
Autumn 2017
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Anna Kolouchová, Ph.D. (assistant)
PharmDr. Katarína Kostolanská (assistant)
Lenka Nerudová (assistant)
Mgr. Lucie Novotná (assistant)
Mgr. Roman Sándor, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Tue 9:20–11:00 A16/213
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Subject is complementary to Medical chemistry lecture (VSLC011p). Students become familiar with the background and equipments of chemical laboratory. They will learn to perform simple laboratory operations. During experiments students verify some findings discussed in theoretical lessons.
Learning outcomes
Students will be able to handle with basal laboratory equipment and follow proper procedures and regulations for safe handling, use, and disposal of chemicals Student will demonstrate understanding - relationship between the composition of different solutions, beverages and body fluids and their pH value
- will be able to describe the general composition and role of the buffers, explain their function and apply this knowledge in explaining the role of buffering systems in the organism
- to describe the essence of the formation of insoluble and poorly soluble compounds, to identify factors that can influence the insolubility of compounds
- will be oriented in relations bettween a structure od bioorganic molecules and their reactivity
Syllabus
  • Safety measures in chemical laboratory. Volumetric ware. Preparation of solutions.
  • Measuring pH of solutions (acids, bases, salts). The construction of titration curves.
  • Preparation of phosphate buffers. Titration of acetate buffer, buffer capacity.
  • Ions in tap water. Reactions of phosphate and carbonate ions.
  • Adsorption chromatography of azodyes (TLC). Column chromatography of carotenoids on aluminum oxide. HPLC (demonstration).
  • Oxidation of alcohols. Determination of alcohol in blood. Detection reactions for aldehydes and ketones. Molecular models of saccharides. Reducing properties of sugars and L-ascorbic acid.
  • Ionization of aminoacids. Colour reactions of aminoacids and proteins. Isoelectric point of casein. Solubility of proteins, precipitation of proteins.
Literature
    required literature
  • Podklady pro cvičení v IS.
Teaching methods
laboratory practicals
Assessment methods
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports is the condition for the course-unit credit. Obtaining of course-unit credits of practicals is the pre-requisite for registration to the examination of Medical chemistry and for enrollment of Biochemistry in the Spring semester.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2017/BVLC011c