ABCHc Biochemistry - practice

Faculty of Medicine
autumn 2019
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Lucie Bláhová, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
RNDr. Marianna Romžová, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Martina Drabinová (assistant)
Martina Hanousková (assistant)
Lenka Nerudová (assistant)
Mgr. Lucie Novotná (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
ABCHc/01: each odd Thursday 8:00–9:40 A16/216, M. Hlaváčová
ABCHc/02: each odd Thursday 8:00–9:40 A16/211
ABCHc/03: each even Thursday 10:00–11:40 A16/212, M. Hlaváčová
ABCHc/04: each even Thursday 10:00–11:40 A16/211
ABCHc/05: each odd Thursday 10:00–11:40 A16/216
ABCHc/06: each odd Thursday 10:00–11:40 A16/211
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Subject is complementary to the Biochemistry lecture ABCHp. Students become familiar with the background and equipments of chemical laboratory. They will learn to perform simple laboratory operations. During experiments students verify some findings discussed in theoretical lessons.
Syllabus
  • Introductory information, safety rules. Making solutions of the given concentration. The measurement of pH of various solutions. Spectrophotometry, calibration curve. Chromatography. Deionization of water using ionex columns. Gel chromatography. Adsorption chromatography of plant pigments (silicagel). HPLC demonstration. Determination of cholesterol in blood. Reflotron, Akutrend. Proteins in blood serum and urine. Electrophoresis of proteins of blood serum. Microalbuminuria by turbidimetry. SDS electrophoresis of proteins. Excursion to clinical chemistry lab.
Literature
    required literature
  • Podklady pro práci ve cvičení jsou uloženy v interaktivní osnově na IS MUNI.
  • Podklady pro cvičení v IS.
Teaching methods
practical exercises, presentation of laboratory methods and instruments.
Assessment methods
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports is the condition for the course-unit credit. Obtaining of course-unit credits of practicals is the pre-requisite for registration to the examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2019/ABCHc