LF:ABCHc Biochemistry-prac - Course Information

ABCHc Biochemistry - practice

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Mgr. David Galuška (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
PharmDr. Kristýna Šebrlová, Ph.D. (seminar tutor)
MUDr. Mgr. Pavel Šmak (seminar tutor)
Iva Audy (assistant)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)

Guaranteed by

MUDr. Michaela Králíková, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

ABCHc/01: Thu 19. 9. 8:00–9:40 A16/212, Thu 3. 10. 8:00–9:40 A16/212, Thu 17. 10. 8:00–9:40 A16/212, Thu 31. 10. 8:00–9:40 A16/212, Thu 14. 11. 8:00–9:40 A16/212, Thu 28. 11. 8:00–9:40 A16/212, Thu 12. 12. 8:00–9:40 A16/212, J. Smutná
ABCHc/02: Thu 19. 9. 8:00–9:40 A16/216, Thu 3. 10. 8:00–9:40 A16/216, Thu 17. 10. 8:00–9:40 A16/216, Thu 31. 10. 8:00–9:40 A16/216, Thu 14. 11. 8:00–9:40 A16/216, Thu 28. 11. 8:00–9:40 A16/216, Thu 12. 12. 8:00–9:40 A16/216, P. Šmak
ABCHc/03: Thu 19. 9. 10:00–11:40 A16/216, Thu 3. 10. 10:00–11:40 A16/216, Thu 17. 10. 10:00–11:40 A16/216, Thu 31. 10. 10:00–11:40 A16/216, Thu 14. 11. 10:00–11:40 A16/216, Thu 28. 11. 10:00–11:40 A16/216, Thu 12. 12. 10:00–11:40 A16/216, D. Galuška
ABCHc/04: Thu 19. 9. 10:00–11:40 A16/211, Thu 3. 10. 10:00–11:40 A16/211, Thu 17. 10. 10:00–11:40 A16/211, Thu 31. 10. 10:00–11:40 A16/211, Thu 14. 11. 10:00–11:40 A16/211, Thu 28. 11. 10:00–11:40 A16/211, Thu 12. 12. 10:00–11:40 A16/211, K. Šebrlová
ABCHc/05: Thu 26. 9. 10:00–11:40 A16/212, Thu 10. 10. 10:00–11:40 A16/212, Thu 24. 10. 10:00–11:40 A16/212, Thu 7. 11. 10:00–11:40 A16/212, Thu 21. 11. 10:00–11:40 A16/212, Thu 5. 12. 10:00–11:40 A16/212, Thu 19. 12. 10:00–11:40 A16/212, K. Šebrlová
ABCHc/06: Thu 26. 9. 10:00–11:40 A16/211, Thu 10. 10. 10:00–11:40 A16/211, Thu 24. 10. 10:00–11:40 A16/211, Thu 7. 11. 10:00–11:40 A16/211, Thu 21. 11. 10:00–11:40 A16/211, Thu 5. 12. 10:00–11:40 A16/211, Thu 19. 12. 10:00–11:40 A16/211, D. Galuška
ABCHc/07: Thu 26. 9. 8:00–9:40 A16/211, Thu 10. 10. 8:00–9:40 A16/211, Thu 24. 10. 8:00–9:40 A16/211, Thu 7. 11. 8:00–9:40 A16/211, Thu 21. 11. 8:00–9:40 A16/211, Thu 5. 12. 8:00–9:40 A16/211, Thu 19. 12. 8:00–9:40 A16/211, D. Galuška, Výuka bude probíhat ve čtvrtek od 8:00

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Biomedicínská technika a bioinformatika (programme LF, C-CV)

Course objectives

Subject is complementary to the Biochemistry lecture and seminars ABCHp and ABCHs. Students become familiar with the background and equipments of chemical laboratory. They will learn to perform simple laboratory operations. During experiments students verify some findings discussed in theoretical lessons.

Learning outcomes

After completing the course, the student will:
- be familiar with safety and health protection at work in the laboratory;
- be able to calculate the concentration of solutions, pipette, prepare or dilute a solution of a given concentration;
- be acquainted with the work with biological material and its processing in the laboratory of clinical biochemistry;
- be able to describe the principles of sampling biological material and its processing
- be familiar with orientation examination of urine using diagnostic strips and with the use of personal glucometers
- be able to work with basic instruments in the biochemical laboratory, eg. pH meters, spectrophotometers, POCT analyzers;
- understand the principle and implementation of selected methods used in clinical biochemistry (optical, chromatographic, electrophoretic, immunochemical methods).

Syllabus

• Introductory information, safety rules. Preparing solutions of the given concentration. Biological material. Examinations in clinical biochemistry and factors influencing the results. Biochemical analyzers. determination of selected analytes in blood and urine using POCT analyzers. The measurement of pH of various solutions. pH-metres. Spectrophotometry, absorption curve. Determination of glucose. Chromatography. Gel chromatography. Adsorption chromatography of plant pigments (silicagel). HPLC demonstration. Electrophoretic and immunochemical methods. Proteins in blood serum and urine. Electrophoresis of blood serum proteins. ELISA.

Literature

required literature
• Podklady pro práci ve cvičení jsou uloženy v interaktivní osnově na IS MUNI.
• Podklady pro cvičení v IS.
• Michaela KRÁLÍKOVÁ, Hana PAULOVÁ a kolektiv autorů. Biochemie pro biomedicínské techniky. 1. vyd. Brno: Masarykova univerzita, 2021. 177 s. ISBN 978-80-210-9857-2

Teaching methods

homework theoretical preparation, practical exercises, presentation of laboratory methods and instruments.

Assessment methods

Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports is the condition for the course-unit credit. Obtaining of course-unit credits of practicals is the pre-requisite for registration to the examination.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 15.

Teacher's information

Attendance in practicals is obligatory. Laboratory coat, laboratory gloves and overshoes are necessary. Student is obliged to prepare himself for each lesson. Students that are not sufficiently prepared will not be admitted in participating in the lesson. All absences must be justified through the Department of study affairs. After being absent the student must make up for the lesson. Student may have only one unexcused absence during the semester.

• Enrolment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2024/ABCHc