LF:BLLM0321c Medical Microbiology I - pr. - Course Information
BLLM0321c Medical Microbiology I - practice
Faculty of Medicineautumn 2020
- Extent and Intensity
- 0/3/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
Alena Svobodová (seminar tutor), MUDr. Ondřej Zahradníček (deputy)
Michaela Gregorovičová (assistant)
Iva Holešovská (assistant)
MUDr. Ondřej Zahradníček (assistant) - Guaranteed by
- prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine - Timetable of Seminar Groups
- BLLM0321c/01: Fri 7:00–9:30 MiÚ N02104
BLLM0321c/02: Fri 11:10–13:40 MiÚ N02104 - Prerequisites
- BLMB011p Mol.andCell.Biol.-lect.
Basic knowledge of laboratory work expected. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Laboratory Technologist (programme LF, B-LABD)
- Laboratory Assistant (programme LF, B-SZ)
- Course objectives
- The aim of the course is to get the students acquainted with the practical implementation of individual methods of medical microbiology and the safe acquisition of these methods.
- Learning outcomes
- At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting - Syllabus
- 1. Safety and health protection in microbiological laboratory. Microscopy - wet mount, simple staining
- 2. Diagnostic Gram staining
- 3. Staining of capsulla, review
- 4. Culure media, preparing blood agar
- 5. Conditions of culture, blood agar culturing, growth of bacteria on culture media
- 6. Evaluation of cultures, biochemical identification tests
- 7. Antibiotic susceptibility testing - disc tests, MIC demostration, E-test demonstration
- 8. Animal experiment
- 9. Introduction to serology - reactions, serum dilution, titer counting, pipetting
- 10. Slide agglutiation, agglutination on carriers
- 11. Agglutination in test tubes, Widal reaction
- 12. Serologic reactions – AKE, RRR
- 13. Complementfixing test
- 14. Neutralisation – ASO
- 15. ELISA
- (16.–30. see BLLM0322c)
- Literature
- recommended literature
- VOTAVA, Miroslav. Lékařská mikrobiologie : vyšetřovací metody. Brno: Neptun, 2010, 495 s. ISBN 9788086850047. info
- Prezentace z přednášek
- Teaching methods
- practical training
- Assessment methods
- For credit, it is necessary:
(1) to absolve all preactical sessions, but:
- two absences, either justified of approved by a teacher are possible; even so student has to demonstrate completing the missing topics
- more absences should be substituted
- not justified absences are not tolerated
(2) complete report of all practicals
(3) succesfully written credit test, eventually also other current tests; information about all tests will be given in advance. - Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Information on completion of the course: Podmínky zápočtu jsou uvedeny v Doplňkových údajích k předmětu
The course is taught annually.
Information on the extent and intensity of the course: 45. - Listed among pre-requisites of other courses
- Enrolment Statistics (autumn 2020, recent)
- Permalink: https://is.muni.cz/course/med/autumn2020/BLLM0321c