MBKM071c Clinical microbiology II - practices

Faculty of Medicine
autumn 2021
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Alena Ševčíková (lecturer)
MUDr. Jana Bednářová, Ph.D. (seminar tutor)
doc. MUDr. Milan Dastych, CSc., MBA (seminar tutor)
MUDr. Markéta Hanslianová (seminar tutor)
MUDr. Ivana Vítková (seminar tutor)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)
Guaranteed by
MUDr. Markéta Hanslianová
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Tue 14. 9. 14:00–15:40 N02255, Tue 21. 9. 14:00–15:40 N02255, Wed 22. 9. 14:00–15:40 N02255, Tue 5. 10. 14:00–15:40 N02255, Tue 12. 10. 14:00–15:40 N02255, Tue 19. 10. 14:00–15:40 N02255, Tue 26. 10. 14:00–15:40 N02255, Tue 2. 11. 14:00–15:40 N02255, Tue 9. 11. 14:00–15:40 N02255, Tue 16. 11. 14:00–15:40 N02255, Tue 23. 11. 14:00–15:40 N02255
Prerequisites
bachelor's studies focused on laboratory medicine or natural sciences
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The subject of Medical Microbiology is based on the already acquired knowledge by students in the Bachelor's degree program.
The aim of the theoretical part is to provide extensive information on the properties of medically important microorganisms and pathogenesis, clinical practice, epidemiology and therapeutics of infectious diseases and detailed information on microbiological investigation procedures, principles of indication of microbiological examinations, proper collection and delivery of infectious material and correct interpretation of the results with respect to the clinical condition of the patient including the possibility of antibiotic therapy. The practical part of the preparation is to gain practical experience and skills in the analysis of biochemical properties, in antigenic, proteomic and molecular biological analysis.
Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases: bacteria, viruses, parasites and yeasts and fungi. It will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. The student will be acquainted with both basic and specialized microbiological procedures, including knowledge of analyzers available in current laboratory microbiological practices. The student will be able to interpret the result of the laboratory examination. Will be trained in combinatory skills, understanding the wider context of infectious diseases. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level. Will be introduced to the principles of quality management in medical microbiology.
Learning outcomes
Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases: bacteria, viruses, parasites and yeasts and fungi. It will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. The student will be acquainted with both basic and specialized microbiological procedures, including knowledge of analyzers available in current laboratory microbiological practices. The student will be able to interpret the result of the laboratory examination. Will be trained in combinatory skills, understanding the wider context of infectious diseases. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level. Will be introduced to the principles of quality management in medical microbiology.
Syllabus
  • Principles of work in the microbiological laboratory (practical application of the principles of anti-epidemic operating rules - disinfection, laboratory accidents containment, first aid), principles of good laboratory practice
  • Microscopy and coloring techniques, both basic and advanced
  • Cultivation soils (preparation of basic and highly specialized cultivation media)
  • Biochemical Identification of Bacteria (inoculation and Reading of Biochemical Tests, Manual and Automatic Identification Systems)
  • Immunological identification of microorganisms
  • Proteomic identification of microorganisms
  • Molecular biological methods for the identification of microorganisms
  • Practical disinfection and sterilization in the laboratory (preparation and control of solutions, work with autoclave)
  • Determination of antibiotic sensitivity and resistance (disk assays, MIC determination)
  • Detection of microbial components in the sample
  • Serological examination (serum dilution, titration, pipetting)
  • Serological techniques - agglutination (agglutination on slide and on carriers, agglutination in tubes - Widal reaction, agglutination in serum platelets)
  • Serological techniques - precipitation (precipitation in gel, flocculation reaction)
  • Serological techniques - complement binding (complement titration, own KFR)
  • Serological techniques - neutralization tests
  • Serological techniques - reactions with labeled components (immunofluorescence, ELISA, Western blotting)
  • Processing of different types of material for bacteriological examination
  • Laboratory diagnostics of individual groups of bacteria (using all available methods)
  • Enterobacteriaceae, campylobacter and helicobacter
  • gram-negative non-fermenting rods, in particular pseudomonads
  • hemophilus, bordetel, neisseria and branhamel
  • staphylococci, streptococci, enterococci and gram-positive rods
  • borrelli and trepon
  • anaerobic microbes
  • tuberculosis
  • Mycological techniques - laboratory diagnostics of yeasts and molds (lye preparation, cultivation, identification), serology in mycology
  • Virological techniques - processing of samples for virus isolation, tissue cultures, molecular biology methods
  • Parasitology techniques - laboratory diagnostics of protozoa (MOP, dg malaria, trichomonoses, lambliosis and toxoplasmosis)
  • Parasitology Techniques - Laboratory Diagnosis of Multicellular Intestinal Parasites (Treatment of Stools for Parasites)
  • Laboratory Information System in the Microbiological Laboratory
  • Good laboratory practice and system of internal and external quality assessment in the microbiological laboratory, validation and verification of methods
Literature
  • JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi : bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011, 73 s. ISBN 9788021056572. info
Teaching methods
Performing and evaluation of practical training. Consultation of gained results with the teacher.
Assessment methods
Credits are given for active participation, attendance and working out quality protocols from individual tasks.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2021, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2021/MBKM071c