LF:MBKM071c Clinical microbiology II - pra - Course Information

MBKM071c Clinical microbiology II - practices

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).

Teacher(s)

MUDr. Alena Ševčíková (lecturer)
MUDr. Jana Bednářová, Ph.D. (seminar tutor)
doc. MUDr. Milan Dastych, CSc., MBA (seminar tutor)
Mgr. Petra Myšková, Ph.D. (seminar tutor)
MUDr. Ivana Vítková (seminar tutor)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)

Guaranteed by

Mgr. Petra Myšková, Ph.D.
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable

Tue 19. 9. 14:00–15:40 N02255, Tue 26. 9. 14:00–15:40 N02255, Tue 3. 10. 14:00–15:40 N02255, Tue 10. 10. 14:00–15:40 N02255, Tue 17. 10. 14:00–15:40 N02255, Tue 24. 10. 14:00–15:40 N02255, Tue 31. 10. 14:00–15:40 N02255, Tue 7. 11. 14:00–15:40 N02255, Wed 8. 11. 14:00–15:40 N02255, Tue 14. 11. 14:00–15:40 N02255, Tue 21. 11. 14:00–15:40 N02255, Tue 28. 11. 14:00–15:40 N02255

Prerequisites

bachelor's studies focused on laboratory medicine or natural sciences

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Bioanalytical Laboratory Diagnostics in Medicine - Bioanalyst (programme PřF, N-BIA)

Course objectives

The subject of Medical Microbiology is based on the already acquired knowledge by students in the Bachelor's degree program.
The aim of the theoretical part is to provide extensive information on the properties of medically important microorganisms and pathogenesis, clinical practice, epidemiology and therapeutics of infectious diseases and detailed information on microbiological investigation procedures, principles of indication of microbiological examinations, proper collection and delivery of infectious material and correct interpretation of the results with respect to the clinical condition of the patient including the possibility of antibiotic therapy. The practical part of the preparation is to gain practical experience and skills in the analysis of biochemical properties, in antigenic, proteomic and molecular biological analysis.
Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases: bacteria, viruses, parasites and yeasts and fungi. It will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. The student will be acquainted with both basic and specialized microbiological procedures, including knowledge of analyzers available in current laboratory microbiological practices. The student will be able to interpret the result of the laboratory examination. Will be trained in combinatory skills, understanding the wider context of infectious diseases. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level. Will be introduced to the principles of quality management in medical microbiology.

Learning outcomes

Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases: bacteria, viruses, parasites and yeasts and fungi. It will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. The student will be acquainted with both basic and specialized microbiological procedures, including knowledge of analyzers available in current laboratory microbiological practices. The student will be able to interpret the result of the laboratory examination. Will be trained in combinatory skills, understanding the wider context of infectious diseases. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level. Will be introduced to the principles of quality management in medical microbiology.

Syllabus

• Principles of work in the microbiological laboratory (practical application of the principles of anti-epidemic operating rules - disinfection, laboratory accidents containment, first aid), principles of good laboratory practice
• Microscopy and coloring techniques, both basic and advanced
• Cultivation soils (preparation of basic and highly specialized cultivation media)
• Biochemical Identification of Bacteria (inoculation and Reading of Biochemical Tests, Manual and Automatic Identification Systems)
• Immunological identification of microorganisms
• Proteomic identification of microorganisms
• Molecular biological methods for the identification of microorganisms
• Practical disinfection and sterilization in the laboratory (preparation and control of solutions, work with autoclave)
• Determination of antibiotic sensitivity and resistance (disk assays, MIC determination)
• Detection of microbial components in the sample
• Serological examination (serum dilution, titration, pipetting)
• Serological techniques - agglutination (agglutination on slide and on carriers, agglutination in tubes - Widal reaction, agglutination in serum platelets)
• Serological techniques - precipitation (precipitation in gel, flocculation reaction)
• Serological techniques - complement binding (complement titration, own KFR)
• Serological techniques - neutralization tests
• Serological techniques - reactions with labeled components (immunofluorescence, ELISA, Western blotting)
• Processing of different types of material for bacteriological examination
• Laboratory diagnostics of individual groups of bacteria (using all available methods)
• Enterobacteriaceae, campylobacter and helicobacter
• gram-negative non-fermenting rods, in particular pseudomonads
• hemophilus, bordetel, neisseria and branhamel
• staphylococci, streptococci, enterococci and gram-positive rods
• borrelli and trepon
• anaerobic microbes
• tuberculosis
• Mycological techniques - laboratory diagnostics of yeasts and molds (lye preparation, cultivation, identification), serology in mycology
• Virological techniques - processing of samples for virus isolation, tissue cultures, molecular biology methods
• Parasitology techniques - laboratory diagnostics of protozoa (MOP, dg malaria, trichomonoses, lambliosis and toxoplasmosis)
• Parasitology Techniques - Laboratory Diagnosis of Multicellular Intestinal Parasites (Treatment of Stools for Parasites)
• Laboratory Information System in the Microbiological Laboratory
• Good laboratory practice and system of internal and external quality assessment in the microbiological laboratory, validation and verification of methods

Literature

• JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi : bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011, 73 s. ISBN 9788021056572. info

Teaching methods

Performing and evaluation of practical training. Consultation of gained results with the teacher.

Assessment methods

Credits are given for active participation, attendance and working out quality protocols from individual tasks.

Language of instruction

Czech

Further comments (probably available only in Czech)

Information on the extent and intensity of the course: 15.

• Enrolment Statistics (autumn 2023, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2023/MBKM071c