MNAB0711s Applied biochemistry

Faculty of Medicine
autumn 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: k (colloquium).
Teacher(s)
MUDr. Lenka Gescheidtová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
MUDr. Ondřej Kyselák, Ph.D., EuSpLM (seminar tutor)
MUDr. Jan Novák, Ph.D. (seminar tutor)
MUDr. Lukáš Opatřil, Ph.D. (seminar tutor)
MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM (seminar tutor)
prof. MUDr. Vladimír Soška, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
prof. MUDr. Dalibor Valík, Ph.D., DABCC (seminar tutor)
Mgr. Ondřej Wiewiorka, Ph.D. (seminar tutor)
MUDr. Ing. David Zeman, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Guaranteed by
prof. MUDr. Vladimír Soška, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: MUDr. Michaela Králíková, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Mon 26. 9. to Fri 23. 12. Mon 15:00–17:50 B11/234; and Mon 12. 9. 15:00–17:50 B11/234, Mon 19. 9. 15:00–17:50 A20/207
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After this course the participant should be able to: - choose biochemical tests suitable for diagnostics of diseases - know the basic problems of common biochemical diagnostic tests - know how to collect biological material and how to treat it to prevent false results - to assess reliability of laboratory results.
Learning outcomes
After this course the participant should be able to: - choose biochemical tests suitable for diagnostics of diseases - know the basic problems of common biochemical diagnostic tests - know how to collect biological material and how to treat it to prevent false results - to assess reliability of laboratory results.
Syllabus
  • Analytical sources of variation, accuracy and precision. Diagnostic accuracy, sensitivity, specificity, and efficiency of diagnostic tests. Reference ranges, decision values. Collection and processing of specimens, special requirements, general safety rules.
  • Assessment and monitoring of nutritional status. Blood plasma proteins. Acute-phase reactants. Principles of nutritional support, enteral and parenteral nutrition. Dietary constituents providing a sufficient energy intake.
  • Energy output, nitrogen balance. Indications for nutritional support. Oxygen, transport and metabolism, tissue hypoxia.
  • Disorders of lipid and lipoprotein metabolism.
  • Ischemic heart disease, congestive cardiac failure. Potassium and magnesium homeostasis.
  • Iron and essential microelements metabolism.
  • Disturbances of water and sodium balance. Plasma and urine osmolality. Plasma electrolytes in acid-base disorders.
  • Assessment of acid-base balance. Investigating an acid-base status, indications for blood acid-base and oxygen measurements, interpretation of results.
  • Laboratory evaluation of kidney function. Biochemical monitoring of acute and chronic renal failure, and of patients receiving haemodialysis. Urine sediment.
  • Investigation of liver and biliary tract diseases. Signs of the liver injury, differentiating of hyperbilirubinaemias, syndrome of biliary obstruction.
  • Clinical biochemistry of the GIT. Gastric acid secretion, maldigestion and malabsorption, assessment of pancreatic function.
  • Disorders of saccharide metabolism, diabetes mellitus.
  • Calcium and phosphate metabolism. Biochemical markers of bone metabolism (in the assessment of osteoporosis).
  • Uric acid, hyperuricaemia. Renal tract stones.
  • Laboratory assessment of thyroid and adrenal functions.
  • Tumour markers. Biological causes of variation of test results, effects of drugs on chemical tests.
Literature
    required literature
  • Klinická biochemie (interaktivní osnova v isu, 41 kapitol, ozvučené prezentace, odpovědníky).
  • RACEK, Jaroslav and Daniel RAJDL. Klinická biochemie. Třetí, přepracované a ro. Praha: Galén, 2021, 454 stran. ISBN 9788074925450. info
  • DASTYCH, Milan. Klinická biochemie - bakalářský obor Zdravotní laborant (Clinical Biochemistry). 1. vydání. Brno: Masarykova univerzita, 2008, 232 pp. ISBN 978-80-210-4572-9. info
    recommended literature
  • Rajdl. D. a kol. Clinical biochemistry (e-book, formats: .pdf, .epub, .mobi) 1st ed. Prague, Karolinum Press, 2016, 426 s. ISBN: 978-80-246-3497-5. Download: https://karolinum.cz/en/books/rajdl-clinical-biochemistry-17096
Teaching methods
seminars
Assessment methods
Colloquium in the form of a written test.
  • The minimum success rate in the test should be at least 60%.
  • The resit test can be taken twice.
    Conditions for participation in the final test:
  • participation in classes. The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Follow-Up Courses
    Further comments (probably available only in Czech)
    The course is taught annually.
    Information on the extent and intensity of the course: 30.
    Listed among pre-requisites of other courses
    The course is also listed under the following terms Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2023, autumn 2024.
    • Enrolment Statistics (autumn 2022, recent)
    • Permalink: https://is.muni.cz/course/med/autumn2022/MNAB0711s