ZLBC0321p Biochemisty I - lecture

Faculty of Medicine
autumn 2023
Extent and Intensity
2/0/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Jana Gregorová, Ph.D. (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
PharmDr. Kristýna Šebrlová, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Thu 13:00–14:50 B11/114
Prerequisites (in Czech)
ZLLC011p Med Chem, Dent Mat - lect && ZLBF0222p Med.Physics and Inf. II - lect && ZLBI0222p Biology II-lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the subject Biochemistry I is to gain knowledge about the basic metabolic processes and pathways taking place at the cellular level.
Understanding these processes is the basis for understanding metabolism at the tissue and organ level, which is studied in the follow-up subject Biochemistry II.
Learning outcomes
At the end of the course, students will:
- understand the meaning of basic chemical concepts and can apply this knowledge to describe biochemical processes in the body and the properties of body fluids.
- discuss the properties and function of enzymes.
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level, and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.
Syllabus
  • Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
Literature
    required literature
  • LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny. 3. vydání. Praha: Univerzita Karlova, nakladatelství Karolinum, 2020, 269 stran. ISBN 9788024647487. info
  • LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny. 3. vydání. Praha: Univerzita Karlova, nakladatelství Karolinum, 2020, Strana 275. ISBN 9788024647494. info
    recommended literature
  • FERRIER, Denise R. Lippincott's illustrated Q&A review of biochemistry. Seventh edition. Wolters Kluwer, 2017. ISBN 9781496344496. info
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Grada. Praha, 2012, 498 pp. ISBN 978-80-247-2977-0. info
Teaching methods
Přednášky.
Assessment methods
Students pass to continuing education in the following semester without any duty of knowledge checking in this semester.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
The course is also listed under the following terms autumn 2020, autumn 2021, autumn 2022, autumn 2024.
  • Enrolment Statistics (autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2023/ZLBC0321p