BLHM0422c Histopathological techniques II

Faculty of Medicine
Spring 2009
Extent and Intensity
0/2/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: z (credit).
Teacher(s)
prof. MUDr. Drahomír Horký, DrSc. (lecturer)
prof. MUDr. Zdeněk Lukáš, CSc. (lecturer)
doc. MUDr. Jitka Šťastná, CSc. (lecturer)
Michaela Gregorovičová (assistant)
Jarmila Jelínková (assistant)
Guaranteed by
prof. MUDr. Jirka Mačák, CSc.
Department of Pathology – Joint workplaces with the University Hospital Brno - workplaces of the Bohunice and Mater. Hospital – Faculty of Medicine
Contact Person: doc. MUDr. Miroslava Sedláčková, CSc.
Timetable
Tue 9:00–12:20 ÚP P01014
Prerequisites (in Czech)
BLHM0321c Histopath. Tech. I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This course is devoted to application of histologic methods in practical pathomorphologic diagnostics (bioptic and necroptic). Student will get to know the practical operation in histopathologic laboratory and obtain skills in demonstration of constituent tissue components by means of histologic, histochemical and immunohistochemical techniques as well as some procedures of molecular biology. Within scope of this course, student will learn recent methods of histopathologic diagnostics of tumors and other pathomorphologic units.
Syllabus
  • Collecting of material for histologic examination. Fixation – physical and chemical fixatives.
  • Dehydration, clearing, embedding into paraffin and celloidin, cutting on sliding and rotary microtomes, cryostat.
  • Staining of histologic sections: types of histologic staining dyes and staining methods. Synoptic staining: hematoxylin – eosine (HE), Weigert-van Gieson, trichromes, AZAN, Mallory staining. Impregnation, evidence of inorganic substances, reticular fibers, pigments.
  • Electron microscope. Tissue collecting for electron microscopy. Fixation for electron microscopy. Embedding, epoxy resins, Durcupan, Araldit. Semithin and ultrathin sections, ultramicrotome. Contrasting.
  • Structure and work of Department of pathology. Laboratory segments within pathology. Equipment of reception office, dispatch note.
  • Types of bioptic collection - biopsy during operation, trepanobiopsy, transport of bioptic material, microexcision, puncture.
  • Adjustment of tissue block, processing machines, registration of samples, time relations for expedition of bioptic findings.
  • Autopsy, structure and working content of autoptic segment of pathology. Didactic segment of pathologic department.
  • Cytodiagnostic, preparation of cytologic smears, cytoblocks, cytospin. Cytologic diagnostics of uterine cervix. Cytologic diagnostics in pneumology, cytologic screening.
  • Histochemistry, basic types of reactions. Collecting of material, fixation, freezing, cryostat sections for histochemical reactions (enzymes, lipids, glycides).
  • Histochemical demonstration of enzymes, basic types of reactions. Evidence of enzymes at malabsorption syndrome and in myopathology.
  • Classification and histochemistry of lipids, basic staining, extraction methods to distinguish polar and apolar lipids. Application – steatosis.
  • Classification and histochemistry of glycides, PAS reaction, staining with alcian blue, application on gastronitestinal tract biopsies. Histochemical demonstration of amyloid, copper and iron.
  • Fundamentals of immunohistochemical reactions, mono- and polyclonal antibodies.
  • Intermedial filaments (cytokeratins, vimentin, desmin, GFAP, neurofilaments), signification for specification of tissue types and their tumorous derivatives. Steps in immunohistochemical diagnostic of tumors.
  • CD antigens and antibodies, application in hematology and in diagnostic of malignant lymphomas.
  • Molecular pathologic methods in diagnostic. Principles of fluorescence in situ hybridization (FISH).
  • Principles of PCR. Advantage and disadvantage of this technique. Differences between immunohistochemical analysis and immunobloting.
Literature
  • Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003, xiii, 796. ISBN 0443064350. info
  • VACEK, Zdeněk. Histologie a histologická technika. 1. vyd. Brno: Institut pro další vzdělávání pracovníků ve zdravotnictví, 1995, 184 s. ISBN 80-7013-202-7. info
Assessment methods
Demonstration and practical implementation of representative histopathologic methods in laboratories of Department of pathology.
The exam is oral, students respond 4 questions selected by lot out of following areas: basic histologic methods and electron microscopy, methods of histochemistry and immunohistochemistry, methods of cytology, methods of molecular pathology.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2009, recent)
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