BLDK061 Laboratory Diagnostic Methods - Clinic

Faculty of Medicine
Spring 2011
Extent and Intensity
0/0/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Prerequisites (in Czech)
BLKG0311 Clinical Genetics && BLKH0311p Clinical Haematology - lecture && BLIM0411p Clin.immunol-lect. && BLKB0422p Clinical Biochemistry II - l. && BLLM0422p Medical Microbiology II - lec. && BLTS0411p Immunohaematology - lec. && BLMP0422 Morphol. and Funct. Pathol. II
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
CLINICAL BIOCHEMISTRY; The goal of the theoretical preparation part is to provide detailed information about methods that utilize the latest knowledge in the field with regard to the contemporary level of routine use of new procedures and technologies. The basics of indication and interpretation of laboratory procedures with regard to clinical units and diagnoses. In the practical preparation part, the goal is to gain practical skills on a level that will allow the graduates to easily be included in clinical laboratory work teams.; CLINICAL HEMATOLOGY; This course includes information about the origin, development, structure and function of the system of hematopoietic cells and organs as well as of the blood clotting system. Both of them are described not only in health but also during various diseases inherited and acquired. Students will receive information summarizing pathophysiologic mechanisms, clinical and mainly laboratory findings in hematologic diseases and laboratory assays serving for treatment’s maintenance. The knowledge will allow students to interpret results obtained during hematological laboratory examination for diagnosis establishing and/or for differential diagnose.; IMMUNOHAEMATOLOGY AND TRANSFUSION SERVICE; The aim of theoretical part of education in immunohaematology is given to understand separate laboratory procedures principles which are currently used in immunohaematology and acquirement of knowledge in relation to its application in laboratory praxis. The accent is putting on ability of separate laboratory results interpretation and/or group laboratory results interpretation to clinical patient hood. Professional theoretical training in HLA covers laboratory testing HLA complex and laboratory results interpretation in relation to solid organs transplant strategy and in relation to strategy of bone marrow donors registry as far as laboratory results interpretation in relation to immunized patients. Theoretical part of education of transfusion service covers background and principles of good manufacturing praxis in preparation, use and quality assurance of transfusion products (allogeneic, autologous) and plasma for fractionation, including quality management and process control, personnel and organisation, equipment and materials, documentation, donor selection, processing, storage and distribution, quality monitoring, quality control, contract management, deviations, complaints, corrective and preventive actions, self inspection, audits and improvement, data processing systems, record keeping, statistical process control, background of safe haemotherapy, principles of haemovigilance system, including types of adverse reactions description and its reporting.; A student will demonstrate the following upon completing the course: Complex theoretical knowledge from the fields of clinical biochemistry, hematology, immunohematology and transfusion service, immunology, microbiology, genetics and pathology;
Syllabus
  • CLINICAL BIOCHEMISTRY; Cardiac disease; Diabetes mellitus; Lipids disorders; Bone metabolism; Disorders of iron metabolism; Disorders of intrinsic milieu; Kidney and urinary tract diseases; Urolithiasis; Gastrointestinal tract diseases; Neoplastic diseases; Endocrinological disorders; Neurological diseases; Reference ranges and cut-off values of laboratory methods; Indication and interpretation of laboratory results; CLINICAL HEMATOLOGY; Origin and development of blood cells.; Basic principles of blood coagulation. ; Plasmatic clotting factors, their physiology. Inherited bleeding disorders.; Primary hemostasis, platelet plug formation and its abnormalities. Basic principles of anti-platelet therapy. ; Inherited thrombophilias, anti-thrombotic treatment and its monitoring. ; Acquired disorders of blood clotting. ; Red cell disorders. ; Myeloproliferative neoplasms.; Acute leukaemias; Myelodyspastic syndrome.; Lymphoproliferative disorders. ; Flow cytometry and molecular genetic assays for diagnosis of hematology diseases. ; Therapeutic aphaeresis, basic principles of hemopoietic cells transplantation. ; IMMUNOHAEMATOLOGY AND TRANSFUSION SERVICE; Safe haemotherapy, ordering and administration, transfusion equipment, uses and contraindications, expected response and potential adverse effects, alternatives to allogeneic transfusion, registry of rare blood group donors; Transfusion management of infants and children, haemolytic disease of foetus and newborn, AB0 and Rh incompatibility, others blood group incompatibility special requirements for intrauterine and neonatal transfusions ; Autoimmune haemolytic anaemia, definition, medical aspects, selection, laboratory testing, safe transfusion, options of haemotherapy ; Acute and delayed transfusion reactions, laboratory findings, clinical evaluation at the bedside; Principles and usage of specific laboratory testing in immunohaematology, absorbing tests, elution techniques, neutralising tests; Quality assurance in immunohaematology; Trouble-shooting and problem-solving in laboratory ; Transplant of bone marrow and haemotherapy blood group conversion ; Infectious complications of transfusion ; Informed consent for transfusion, lookback and recipient notification and product recall and withdrawls ; Haemovigilance system, transfusion committee, process improvement;
Literature
  • DASTYCH, Milan. Klinická biochemie - bakalářský obor Zdravotní laborant (Clinical Biochemistry). 1. vydání. Brno: Masarykova univerzita, 2008, 232 pp. ISBN 978-80-210-4572-9. info
Teaching methods
Reviewing the subjects from the courses from clinical laboratory fields; Consultation for the selected subjects; Self-study according to the syllabus of the subjects being examined;
Assessment methods
Oral final state examination before a board of examiners; Defence of the bachelor’s thesis; final state examination
Language of instruction
Czech
The course is also listed under the following terms Spring 2008, Spring 2009, Spring 2010.
  • Enrolment Statistics (recent)
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