BLIT063 Laboratory Instrument and Analytic Technology

Faculty of Medicine
Spring 2011
Extent and Intensity
0/0/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Prerequisites (in Czech)
BLKG0311 Clinical Genetics && BLKH0311p Clinical Haematology - lecture && BLIM0411p Clin.immunol-lect. && BLKB0422p Clinical Biochemistry II - l. && BLLM0422p Medical Microbiology II - lec. && BLTS0411p Immunohaematology - lec. && BLMP0422 Morphol. and Funct. Pathol. II
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of the theoretical preparation part is to provide a theoretical base of physical principles used in analytical and laboratory technology. To familiarize students in detail with the application of these physical principles in specific instruments including existing modifications in contemporary medical laboratory equipment. To observe variations and modifications of specific examples and determine their purpose, advantages and critical sections in seminars, demonstrations and practical exercises.; Course aims A student will demonstrate the following upon completing the course: Complex theoretical knowledge of instrumental and analytical equipment used in the fields of clinical biochemistry, hematology, immunohematology and transfusion service, immunology, microbiology, genetics and pathology;
Syllabus
  • Basic physical principals used in instrumental technology; Optical methods (spectrophotometry, turbidimetry, fluorimetry, nephelometry, denzitometry, refractometry, reflectance photometry, phlame emission photometry, atomic absorption spectrophotometry; Electrochemical methods (potentiometry, amperometry, polarography, coulometry, conductometry, electrophoresis); Ion-selective electrodes (ISE); Kryoscopy, ebullioscopy; Microscopy; Radioactive isotopes; Centrifugation; Basic physical principals used in instrumental technology – Demonstration; Spectrophotometer-design (sources of lightradiation, monochromatores, interference filtrs, cuvettes, detectors; Phlame emission photometer -design, sampling of analytical sample, internal standard; pH meter; Polarograph; Coulometer; Conductometer; Denzitometer; Application of basic physical principals in laboratory instrumentation; Spectrophotometer UV/VIS, fluorimeter, nephelometer, denzitometer, refractometer, reflectance photometer, phlame emission photometer, atomic absorption spectrophotometer; pH meter, polarograph, coulometer, conductometer; electrophoretic device (agarose, polyakrylamid, isoelectric focusation); Thin layer chromatography device, liquid chromatograph-HPLC, gas chromatograph-GC; Osmometer; ISE (Na, K, Cl, Ca, Mg); Clarck electrode (pO2), Severinghaus electrode (pCO2 ), enzymatic electrodes; Centrifuges; Termostats, incubation bathes; Microwave mineralization device; Devices for RIA; Luminometer;
Literature
  • DASTYCH, Milan. Instrumentální technika : obor zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2007, 131 s. ISBN 9788021042261. info
Teaching methods
Reviewing the instrumental subjects from the courses from clinical laboratory fields; Consultation for the selected subjects; Self-study according to the syllabus of the subjects being examined;
Assessment methods
Oral final state examination before a board of examiners; Defence of the bachelor’s thesis; final state examination
Language of instruction
Czech
The course is also listed under the following terms Spring 2008, Spring 2009, Spring 2010.
  • Enrolment Statistics (recent)
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