BLBC0211c Biochemistry - practice

Faculty of Medicine
Spring 2018
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Anna Kolouchová, Ph.D. (assistant)
PharmDr. Katarína Kostolanská (assistant)
Lenka Nerudová (assistant)
Mgr. Lucie Novotná (assistant)
Mgr. Roman Sándor, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: Lenka Nerudová
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Thu 7:30–10:00 A16/211
Prerequisites (in Czech)
BLLC0111s Med.Chem.-seminar && ZC011 Handling chemical substances && BLLC0111c Medical Chemistry -p
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Subject is complementary to subject BLBC021p. At the end of the course student understands metodical principles and procedures used in clinical biochemistry for determination analytes and enzyme activities in various body fluids.Knows the principles of work with biological material. Is able to suggest and organize laboratory experiment. Interprets the result of laboratory test.
Learning outcomes
At the end of the course student understands metodical principles and procedures used in clinical biochemistry for determination analytes and enzyme activities in various body fluids.Knows the principles of work with biological material. Is able to suggest and organize laboratory experiment. Interprets the result of laboratory test
Syllabus
  • Handling biological materiál and cheical substances. Laboratory examinations. Blood collection and processing. --- Enzymic determination of blood plasma glucose. Using personal glucometer. Oral glucose tolerance test(oGTT). Urine test fro glucose and ketone bodies. Assay for glycated hemoglobin HbA1c. --- Enzymic method for serum cholesterol. Determination of blood cholesterol by Accutrend. Determination of blood lipids by Accutrend. Determination of HDL-/LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. --- Determination of catalytic concentration of ALT, AST in serum. Determination of catalytic concentration of ALP in serum. --- Determination of total bilirubin in serum. Urine test form bilirubin and urobilinogen. Determination of albumin in serum. --- Determination of total serum proteins. Determination of serum albumin. Elektrophoresis of serum proteins. Assay for CRP. Determination of serum IgE by ELISA method. --- Investigation of urine. Determination of creatinine in serum and urine. Determination of glomerular filtration from creatinine clearance. Fractional reabsorption and excretion of water. --- Detection of proteinuria. Urine test form hemoglobin. Detection of microalbuminuria. Detection of Bence-Jones protein in urine. Determination of urean in serum and urine. Approximate assesment of nitrogen balance. --- Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. --- Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. --- Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract. --- Determination of troponin T in blood. Determination of catalytic concentration of creatine kinase in serum. Determination of mass concentration of creatine kinase in serum. Determination of myoglobin in blood.
Literature
    required literature
  • Aktuální návody ke cvičením jsou k dispozici ve Studijních materiálech předmětu v IS.
Teaching methods
Practical work in laboratory.
Assessment methods
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports and handing them to the teacher till the given data. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry I.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2018, recent)
  • Permalink: https://is.muni.cz/course/med/spring2018/BLBC0211c