MBKI081c Clinical Imunology II - practices

Faculty of Medicine
spring 2019
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Marcela Vlková, Ph.D. (seminar tutor)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)
Guaranteed by
Mgr. Marcela Vlková, Ph.D.
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable
Tue 5. 3. 13:00–15:30 ÚKIA N01021, Tue 12. 3. 13:00–15:30 ÚKIA N01021, Tue 19. 3. 13:00–15:30 ÚKIA N01021, Tue 26. 3. 13:00–15:30 ÚKIA N01021, Tue 2. 4. 13:00–15:30 ÚKIA N01021, Tue 9. 4. 13:00–15:30 ÚKIA N01021, Tue 16. 4. 13:00–15:30 ÚKIA N01021, Tue 23. 4. 13:00–15:30 ÚKIA N01021
Prerequisites
Bachelor's studies focused on laboratory medicine or natural sciences
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in exercises focuses on methods used in immunological laboratory diagnostics of immunopathological conditions, on indication and interpretation of laboratory immunological laboratory examinations and on investigation algorithms in the diagnosis of immunopathological conditions.
Learning outcomes
Upon completion of the course the student will be able to perform and interpret basic and special laboratory immunological methodologies. The student must be able to prepare the laboratory protocols for the laboratory tests.
Syllabus
  • Collection, reception, basic processing and preservation of biological material before the examination.
  • Laboratory Information System in Immunological Laboratory. Performance reporting, economic monitoring.
  • Management of laboratory protocols.
  • Qualitative and quantitative determination of immunoglobulins in serum and other biological materials.
  • Qualitative and quantitative determination of paraproteins and cryoglobulins.
  • Detection of natural and postvaccination antibodies.
  • Determination of acute phase proteins.
  • Determination of components and functional activity of the complement system.
  • Immunophenotyping of lymphocytes, monocytes, other cells on the flow cytometer.
  • Functional tests of lymphocytes (proliferation after stimulation of mitogens and antigens, evidence of cytokine formation, cytotoxicity).
  • Functional examination of professional phagocytes (chemotaxis, ingestion, respiratory flare).
  • Laboratory diagnosis of allergic diseases: determination of total and specific IgE, precipitation of extrinsic alveolitis.
  • Laboratory diagnosis of autoimmune diseases: examination of autoantibodies by immunofluorescence and immunoenzymatic techniques.
  • Selected molecular-genetic methods for the diagnosis of immunological diseases.
  • Immunization and blood collection in experimental animals.
  • Utilization of PC for the acquisition, processing and presentation of data from laboratory immunology.
Literature
  • Dopl.
Teaching methods
Performence, writing and evaluation of laboratory tasks. Consultation of findings with the teacher.
Assessment methods
Credits are obtained after active participation of the practices, finished protocols and examination of gained knowledge and practical skills
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2019, recent)
  • Permalink: https://is.muni.cz/course/med/spring2019/MBKI081c