EMBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Mon 8:00–10:30 A16/216
Prerequisites (in Czech)
EMBF0211c Biophysics - practice && EMBC0321s Biochemistry I - seminar && EMFY0321c Physiology I - practicals && EMFY0321s Physiology I - practicals
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to the course EMBC0422p. The aim of the course is to acquaint students with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and methods of interpretation of laboratory results.
Learning outcomes
After completing the course, the student will be able to:
- follow the principles of working with biological material
- be familiar with basic laboratory tasks
- explain methods of obtaining biological material for biochemical analyzes
- describe the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids
- interpret the results of biochemical analyzes, discuss the significance of the findings
Syllabus
  • Organization of work in practical exercises. Handling with biological material.
  • Blood collection and processing.
  • Pipetting.
  • Communication of biochemical test results.
  • Determination of plasma glucose, determination of glycemia by personal glucometer. Oral glucose tolerance test (oGTT). Evidence of glucosuria and ketonuria. Determination of glycated hemoglobin.
  • Determination of total cholesterol in serum and blood. Determination of triacylglycerols in blood. Determination of HDL- and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. Determination of catalytic concentrations of aminotransferases in serum.
  • Determination of total serum bilirubin. Detection of bilirubin and urobilinogens in urine.
  • Determination of serum albumin.
  • Determination of total protein and serum albumin. Serum protein electrophoresis.
  • Detection and determination of C-reactive protein in serum and blood. Determination of ferritin in serum.
  • Objective and physical examination of urine.
  • Determination of creatinine in serum and urine. Determination of glomerular filtration from creatinine clearance. Calculation of fractional water resorption and excretion.
  • Proof and determination of proteinuria and albuminuria. Evidence of blood pigment in the urine. Detection of Bence-Jones protein in urine.
  • Determination of urea in serum and urine. Nitrogen balance.
  • Examination of urine with multifunctional diagnostic strips. Demonstration of drugs in urine. Detection of luteinizing hormone in urine.
  • Solubility of uric acid. Orientational chemical analysis of urinary stones.
  • Determination of uric acid in serum and urine.
  • Selected laboratory examinations of the stomach. Laboratory tests for pancreatic diseases Digestive tract occult bleeding test.
Literature
    required literature
  • Návody ke cvičením dostupné v IS MUNI.
Teaching methods
Lab exercises
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all lessons,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI,
  • successful completion of the credit test (at least 70% of points).
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu EMBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    https://is.muni.cz/auth/el/1411/jaro2022/EMBC0422c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms spring 2023, spring 2024, spring 2025.
    • Enrolment Statistics (spring 2022, recent)
    • Permalink: https://is.muni.cz/course/med/spring2022/EMBC0422c