EMBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2023
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Fri 11:00–13:30 A16/216
Prerequisites (in Czech)
EMBF0211c Biophysics - practice && EMBC0321s Biochemistry I - seminar && EMFY0321c Physiology I - practicals && EMFY0321s Physiology I - practicals
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to the course EMBC0422p. The aim of the course is to acquaint students with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and methods of interpretation of laboratory results.
Learning outcomes
After completing the course, the student will be able to:
- follow the principles of working with biological material
- be familiar with basic laboratory tasks
- explain methods of obtaining biological material for biochemical analyzes
- describe the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids
- interpret the results of biochemical analyzes, discuss the significance of the findings
Syllabus
  • Introduction to practical exercises - organization, principles of work and safety, principles of pipetting. Blood, laboratory examination
  • Selected examinations of glucose metabolism - P/U-glucose, OGTT, U-ketone bodies, S-HbA1
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol.
  • Selected liver examinations - S-ALT/AST, S/U-bilirubin, U-UBG, S-albumin
  • Selected examinations of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S-CRP, S-ferritin
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, U-blood, S/U-urea, nitrogen balance
  • Chemical qualitative analysis of urine - diagnostic strips
  • Laboratory diagnosis of myocardial infarction - B-troponin T, diagnostic accuracy
  • Selected examinations of urolithiasis - S/U-uric acid, indicative chemical analysis of stones
Literature
    required literature
  • Studijní podklady a materiály k předmětu dostupné v IS MUNI.
Teaching methods
Lab exercises
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes, missed exercises must be made up,
  • elaboration and approval of all protocols,
  • completion of the final test (at least 70% success rate) or the credit test (at least 60% success rate).
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu EMBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    https://is.muni.cz/auth/el/1411/jaro2024/EMBC0422c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms spring 2022, spring 2024, spring 2025.
    • Enrolment Statistics (spring 2023, recent)
    • Permalink: https://is.muni.cz/course/med/spring2023/EMBC0422c