FaF:FABL2_16 Biotechnology of Drugs-practic - Course Information

FABL2_16 Biotechnology of Drugs - practical classes

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Marie Brázdová, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)

Guaranteed by

PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Prerequisites

3120/FAME1 Molecular Biology I
3120/FAMG1 Methods of Molecular Biology
3120/FABL1 Biotechnology of Drugs

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARMA)

Course objectives

This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.

Learning outcomes

Basic knowledge in methods of pharmaceutical biotechnology

Syllabus

• 1) Isolation of plant chromosomal DNA
  2) Amplification of selected plant gene by polymerase chain reaction
  3) Purification of PCR products
  4) Isolation of plasmid DNA
  5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
  6) Digestion of vector DNA by restriction endonucleases
  7) Ligation of PCR product into a vector
  8) Transformation of bacterial cells Escherichia coli
  9) PAGE

Literature

required literature
• Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
• Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info

not specified
• Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info

Teaching methods (in Czech)

Laborování

Assessment methods (in Czech)

Rozbor produktů pracovní činnosti studenta (technické práce)

Language of instruction

English

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.

Teacher's information

100% attendance at compulsory exercises
laboratory protocols
final test

Activity	Difficulty [h]
Domácí příprava na výuku	5
Účast na výuce	30
Summary	35

• Enrolment Statistics (Spring 2020, recent)
  
• Permalink: https://is.muni.cz/course/pharm/spring2020/FABL2_16