F1PB1_16 Pathobiochemistry

Faculty of Pharmacy
Spring 2025
Extent and Intensity
2/1/0. 4 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
Mgr. Bc. Daniela Nykodýmová (lecturer)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (lecturer)
Mgr. Petra Herczogová (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (lecturer)
PharmDr. Zuzana Soldánová (lecturer)
Mgr. Ondřej Smištík (seminar tutor)
Guaranteed by
Mgr. Marie Brázdová, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Prerequisites
The knowledge of subject in range of lectures
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 72 student(s).
Current registration and enrolment status: enrolled: 0/72, only registered: 0/72, only registered with preference (fields directly associated with the programme): 0/72
fields of study / plans the course is directly associated with
Course objectives
Pathobiochemistry follows the course of Biochemistry taught in the winter semester. Biochemistry deals with the metabolic processes in living organisms.
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy.
The aim of the practical exercises are analyzes of some selected diagnostically important compounds, the correct interpretation of the results of analyzes and their use for the purpose of human diagnostic and therapeutic practice.
Learning outcomes
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy. Advance knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology and Pharmacogenomics.
Syllabus
  • Syllabus Pathobiochemistry

    1. Introduction, the importance of studying pathobiochemistry. The scope and requirements for successful completion of the course exam, recommended literature. Understanding the regulation of metabolism. Biochemical communication.
    2. Metabolism disorders, types and causes. Hereditary metabolic diseases. Enzymes, regulation of metabolism. Causes increased activity of cellular enzymes in the plasma. Clinically significant enzymes.
    3. Pathobiochemistry of carbohydrates, glucose metabolism and its disorders. Glycemic control disorders. Pathobiochemistry of diabetes mellitus, types of DM. Disorders of glycogen metabolism, glykogenosis.
    4. Amino acid metabolism and its disorders. Types of diseases and therapy.
    5. Disorders of lipid metabolism. Cholesterol, lipoproteins. Lipidosy, dyslipoproteinaemia.
    6. Pathobiochemistry of arteriosclerosis. Ischemic heart failure - cardiac markers.
    7. The nucleic acid metabolism disorders of purine and pyrimidine. Hyperuricemia, orotacidurie, therapy.
    8. Blood, blood plasma proteins. Blood clotting, coagulopathy. Dysproteinaemias. Porphyrins. Biosynthesis, metabolism disorders. Porphyria, hemoglobinopathies.
    9. Xenobiotics and their effects on the body. Detoxification mechanism. Biological oxidation. The effects of free radicals on the organism. Lipoperoxidation antioxidants.
    10. Tumor, tumor markers. Basic characteristics of tumor cells. Strategy laboratory tests. Requirements ideal tumor marker. Used tumor markers.
    11. Relation between Pathobiochemistry and Clinical Biochemistry. Clinical and biochemical analysis and its specific features. Terminology of Clinical Biochemistry. The analyzed material. Material removal.
    12. Analysis of urea and the urinary sediment. Immunochemical methods.
    13. Hormones and neurodegenerative disorders. 14. Inflamation.
    Sylabus of practical exercises in pathobiochemistry:
    1. Exercise: Analysis of tumor suppressor by immunodetection on the membrane.
    2. Exercise: Basic biochemical determinations.
    3. Exercise: Immunochemical methods.
    4. Exercise: Hematological methods. Use of automated hematology analyzers in clinical practice. Coagulation methods.
    5. Exercise: Final test
    Guarantors: Mgr. Marie Brázdová, Ph.D.,
    Tutorial lecturers:
    Mgr. Marie BRÁZDOVÁ, Ph.D.
    , Mgr. M. Peter, Mgr. Adamík
    Lecturers:
    Mgr. Marie BRÁZDOVÁ, Ph.D.
    Mgr. Z. Soldánová
    Garant of discipline:
    Mgr. Marie Brázdová, PhD.

Literature
    required literature
  • G.F,.Hoffmann et al. Dědičné metabolické poruchy. Grada,, 2012. info
    recommended literature
  • J.Koolman, K.H.Rohm. Barevný atlas biochemie. Grada, 2012. info
  • Murray et al. Harpeŕ´s Illustrated Biochemistry. 29th Edition. Lange, 2012. info
  • Robbins SL, Cotrans RS, Kumar V. The Pathologic Basis of Disease, 3rd Edition. Saunders, 2010. info
  • Ledvina a kol. Biochemie pro studující medicíny - I. a II.díl. Karolinum, 2005. info
  • KARLSON, P., GEROK, W., GROSS, W. Pathobiochemie. Academia, Praha, 1987. info
Teaching methods
lectures, class discussion, laboratory work, video
Assessment methods
The written part (online), which consists of a on-line test. . To successfully complete the course, it is necessary to pass the test at 60%. Prerequisite for passing the exam is to complete all practical exercises, passing a test from practical work at 80%, adequate knowledge of biochemical issues and the correct elaboration of all protocols for exercises. He will then reimburse the teaching at the time and in the manner determined by the respective teacher.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Prerequisite for granting credit from the subject Pathobiochemistry is the completion of all practical exercises and appropriate knowledge of exercises and lectures. Knowledge control is performed in writing (online).
The course is also listed under the following terms Spring 2020, Spring 2021, Spring 2023, Spring 2024.
  • Enrolment Statistics (Spring 2025, recent)
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